Research ResourcesIMMUNOTHERAPY

In-depth tissue profiling using multiplexed immunohistochemical consecutive staining on single slide

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Science Immunology  14 Jul 2016:
Vol. 1, Issue 1, pp. aaf6925
DOI: 10.1126/sciimmunol.aaf6925

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Putting immune cells on the map

Multiplexed flow cytometry allows for careful characterization of the complexity of immune cells. However, these cells do not act in isolation, and multiplexed analyses do not extend to spatial visualization of cells in tissue microenvironments. Remark et al. have combined immunohistochemistry with virtual landscaping to generate maps of immune cells in tumors stained with up to 10 distinct antibodies. Rather than using filters to resolve chromogens, they use multiple rounds of staining and destaining, followed by virtual color assignment. This simple yet powerful technique can be used to chart maps of cells in any tissue.