Table 1 Affinity measurements.

Kdeq is in μM; kon is in M−1 s−1 × 104; koff is in s−1; t1/2 is in s. ND, not determined. The error is representative of the SD of the experiment performed in duplicate from at least two independent experiments (n ≥ 2).

KdeqDR1-Gag293*DR1-RQ13DR11-Gag293*DR11-RQ13DR15-Gag293DR15-RQ13DRB5-Gag293*DRB5-RQ13
F246.97 ± 0.2210.56 ± 2.620.86 ± 0.151.16 ± 0.485.09 ± 0.456.90 ± 0.622.58 ± 0.192.49 ± 0.80
F2551.50 ± 3.00153.4 ± 10.653.58 ± 0.255.36 ± 1.41152.0 ± 15.56159.6 ± 29.6916.00 ± 0.6037.28 ± 2.02
F5>100173.0 ± 9.9011.06 ± 1.7314.33 ± 3.18122.0 ± 14.1474.48 ± 13.3474.45 ± 6.2575.6 ± 4.53
konDR1-Gag293DR1-RQ13DR11-Gag293DR11-RQ13DR15-Gag293DR15-RQ13DRB5-Gag293DRB5-RQ13
F241.15 ± 0.541.28 ± 0.2410.00 ± 0. 3010.41 ± 0.794.95 ± 0.034.50 ± 0.055.29 ± 0.544.54 ± 0.09
F25NDND9.14 ± 1.365.42 ± 0.07NDNDND0.21 ± 0.02
F5NDND1.51 ± 0.070.89 ± 0.03NDNDNDND
koffDR1-Gag293DR1-RQ13DR11-Gag293DR11-RQ13DR15-Gag293DR15-RQ13DRB5-Gag293DRB5-RQ13
F240.418 ± 0.0130.172 ± 0.0160.062 ± 0.0020.050 ± 0.0010.142 ± 0.0060.153 ± 0.0080.179 ± 0.0090.167 ± 0.003
F25NDND0.253 ± 0.0050.113 ± 0.004NDNDND0.498 ± 0.076
F5NDND0.160 ± 0.0450.107 ± 0.001NDNDNDND
t1/2DR1-Gag293DR1-RQ13DR11-Gag293DR11-RQ13DR15-Gag293DR15-RQ13DRB5-Gag293DRB5-RQ13
F241.64.011.111.84.84.53.84.1
F25NDND2.76.1NDNDND1.4
F5NDND4.36.5NDNDNDND

*Kdeq values previously published (10).