Research ArticleVACCINES

Dendritic cell–targeted lentiviral vector immunization uses pseudotransduction and DNA-mediated STING and cGAS activation

See allHide authors and affiliations

Science Immunology  21 Jul 2017:
Vol. 2, Issue 13, eaal1329
DOI: 10.1126/sciimmunol.aal1329

Successful vaccination through “pseudo” science

Successful vaccines need to deliver antigen as well as stimulate the immune response. Kim et al. examine the mechanisms by which dendritic cell (DC)–targeting lentiviral vectors (LVs) accomplish both of these goals. They found that transduction was not required and that pseudotransduction was sufficient for antigen delivery and immune stimulation. The authors identified two mechanisms behind this effect: Viral fusion induced a PI3K-dependent immune stimulation pathway, and human genomic DNA within the virion preparations activated the STING/cGAS pathway. These data suggest that STING and cGAS agonists could serve as potential vaccine adjuvants.

View Full Text

Stay Connected to Science Immunology