Technical CommentRHEUMATOID ARTHRITIS

Technical comment on “Synovial fibroblast-neutrophil interactions promote pathogenic adaptive immunity in rheumatoid arthritis”

See allHide authors and affiliations

Science Immunology  31 Jan 2020:
Vol. 5, Issue 43, eaax5672
DOI: 10.1126/sciimmunol.aax5672

Tables

  • Table 1 Identified sites of citrullination.

    r, citrulline; c, alkylated cysteine; m, oxidized methionine; n or q, deamination; m/z, mass/charge ratio; IO, ionophore; RF, rheumatoid factor.

    ProteinModified argininePeptide sequenceChargem/zPred. m/zNET ind.
    ACTBP60709183(R)LDLAGrDLTDYLMK(I)2812.9162812.9162IO
    196(K)ILTErGYSFTTTAER(E)2873.4393873.4389RF
    210(R)GYSFTTTAErEIVR(D)3544.2738544.2739RF
    2815.9070815.9072RF
    256(R)FrcPEALFQPSFLGMEScGIHETTFNSIMK(C)31179.21351179.2100IO
    312(K)DLYANTVLSGGTTMYPGIADrmQK(E)3868.4185868.4209IO/RF
    ACTCP6803230(K)AGFAGDDAPrAVFPSIVGRPR(H)3719.7115719.7115RF
    39(R)AVFPSIVGrPRHQGVMVGMGQK(D)4588.8162588.8161RF
    256(K)SYELPDGQVITIGNErFR(C)31048.02251048.0264RF
    2699.0182699.0200RF
    ARPC3O1514534(K)GPAPrETKDTDIVDEAIYYFK(A)3810.4000810.4006IO
    CDDP323205(K)rPAcTLKPEcVQQLLVcSQEAK(K)3873.7701873.7696IO
    21308.64921308.6508IO
    COR1AP3114612(K)FrHVFGQPAKADQcYEDVR(V)4581.7795581.7774IO
    416(R)rAAPEASGTPSSDAVSRLEEEMR(K)3816.3864816.3871IO/RF
    453(K)RLDrLEETVQAK(−)2729.8989729.8992IO
    CZIBQ9NWV458(R)LMDSVALKGGrGSASMVQK(C)3646.0028646.0045IO
    ELMO1Q92556405(R)IVLENSSrEDKHEcPFGR(S)4544.2626544.2628RF
    ERHP8409096 and 97(K)IYVLLrrQAQQAGK(−)2823.4727823.4728IO
    EZR1P15311393(R)AQEEAERLEADrMAALR(A)3653.9898653.9899IO
    GDIR1P5256549(K)SIQEIQELDKDDESLrK(Y)3683.0105683.0112IO
    HMGB1/2P0942924(K)MSSYAFFVQTcrEEHKK(K)3716.9998716.9996IO
    (K)mSSYAFFVQTcrEEHKK(K)3722.3307722.3312RF
    HNRPUQ00839347(K)VTEKIPVrHLYTK(D)4396.9828396.9832IO
    558(K)LNTLLQrAPQcLGK(F)2806.9442806.9456IO
    601(K)McLFAGFQrK(A)2629.8071629.8072IO
    HP1B3Q5SSJ5269(K)LEDVLPLAFTrLcEPK(E)3634.6734634.6729IO
    293 and 297(K)LrVDIrPQLLK(N)2676.9167676.9166IO
    HSP90AP07900355(R)APFDLFENrK(K)2619.3152619.3142IO
    LDHA/BP00338106/107(R)QQEGESrLNLVQR(N)2779.4013779.4026IO
    LKHAP09960587(K)DLAAFDKSHDQAVrTYQEHK(A)4590.7869590.7871IO
    MOESP26038408(R)QEAEEAKEALLQASrDQK(K)3682.3433682.3430IO
    460(K)AQMVQEDLEKTrAELK(T)3630.6595630.6594RF
    533(K)ALTSELANArDESKK(T)3545.2847545.2846IO
    MPO H14P05164219(K)rNGFPVALAR(A)2551.3118551.3118IO
    MYH9P355791922 and 1923(K)LrrGDLPFVVPR(R)3476.2770476.2770RF
    1191(K)TLEEEAKTHEAQIQEMrQK(H)3767.3764767.3778IO
    1751(R)IAQLEEELEEEQGNTELINDrLK(K)3948.1448948.1487IO
    1830(K)IAQLEEQLDNETKErQAAcK(Q)3792.3874792.3884IO
    1912(R)ELEDATETADAMNrEVSSLK(N)3737.3418737.3425IO
    NCF1P1459885(K)WFDGQrAAEnRQGTLTEYcSTLMSLPTK(I)31088.17521088.1763IO
    85 and 90(K)WFDGQrAAENrQGTLTEYcSTLMSLPTK(I)31088.17611088.1763IO
    340(R)RqArPGPQSPGSPLEEER(Q)3664.9952664.9960IO
    PGM1P36871452(K)DLEALMFDrSFVGK(Q)3543.6045543.6045RF
    2814.9048814.9031IO
    PLSLP137963(M)ac-ArGSVSDEEMMELR(E)2826.8567825.8664IO
    ROA1P09651232(R)SrGFGFVTYATVEEVDAAMNARPHK(V)4689.3417689.3381IO
    ROA2P2262612(K)TLETVPLErK(K)3396.2277396.2274IO
    S100-A8P0510947(K)LLETEcPQYIrKK(G)3560.2991560.2993IO
    (K)LLETEcPQYIrK(K)2775.8961775.8978IO
    S10A4P2644749(R)ELPSFLGKrTDEAAFQK(L)3646. 6691646.6707IO
    SH3L3Q9H29915(R)VYSTSVTGSrEIKSQQSEVTR(I)3781.7313781.7311IO
    (R)VYSTSVTGSrEIK(S)2714.3721714.3725IO
    51(R)IQYQLVDISQDNALRDEMrALAGNPK(A)3987.1703987.1696IO
    SPTB2Q010821307(K)MLTAQDmSYDEArNLHSK(W)3709.6548709.6542IO
    TBA4AP6836621 and 23(K)EIIDPVLDrIrK(L)2734.9221734.9221IO
    TBB4B/5P68371162(K)IREEYPDrIMNTFSVVPSPK(V)3793.3715793.7386IO/RF
    TLN1Q9Y4902538(K)FLPSELrDEH(−)2622.3012622.3013RF
    1625(R)ALAVNPrDPPSWSVLAGHSR(T)3711.0381711.0461IO
    VIMEP08670145(K)SrLGDLYEEEMRELR(R)3500.2326500.2329IO
    2948.9588948.9596IO
    304(K)FADLSEAANrNNDALR(Q)2889.4271889.4268RF

Stay Connected to Science Immunology

Navigate This Article