Research ArticleALLERGY

Origins and clonal convergence of gastrointestinal IgE+ B cells in human peanut allergy

See allHide authors and affiliations

Science Immunology  06 Mar 2020:
Vol. 5, Issue 45, eaay4209
DOI: 10.1126/sciimmunol.aay4209

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution

Peanuts in the gut

Peanut allergy affects millions of children and adults worldwide, and peripheral blood IgE+ B cells have been studied in previous peanut allergy studies. Less is known about B cells in tissues exposed to peanuts, and Hoh et al. have now characterized IgE+ B cell clones in the blood, esophagus, stomach, and duodenum in 19 peanut-allergic patients. The stomach and duodenum were enriched for IgE+ B cells with a plasma cell phenotype in allergic patients. High-throughput DNA analysis indicated that tissues were the sites of local isotype switching, and similar antibody sequences for the Ara h 2 peanut allergen were shared between patients. These data better define a role for IgE+ B cells in the gastrointestinal tract in peanut allergy.

Abstract

B cells in human food allergy have been studied predominantly in the blood. Little is known about IgE+ B cells or plasma cells in tissues exposed to dietary antigens. We characterized IgE+ clones in blood, stomach, duodenum, and esophagus of 19 peanut-allergic patients, using high-throughput DNA sequencing. IgE+ cells in allergic patients are enriched in stomach and duodenum, and have a plasma cell phenotype. Clonally related IgE+ and non-IgE–expressing cell frequencies in tissues suggest local isotype switching, including transitions between IgA and IgE isotypes. Highly similar antibody sequences specific for peanut allergen Ara h 2 are shared between patients, indicating that common immunoglobulin genetic rearrangements may contribute to pathogenesis. These data define the gastrointestinal tract as a reservoir of IgE+ B lineage cells in food allergy.

View Full Text

Stay Connected to Science Immunology