Oral epithelial IL-22/STAT3 signaling licenses IL-17–mediated immunity to oral mucosal candidiasis

See allHide authors and affiliations

Science Immunology  05 Jun 2020:
Vol. 5, Issue 48, eaba0570
DOI: 10.1126/sciimmunol.aba0570

Type 17 tag team

Effective immune defense against invasive oral infections by the fungus Candida albicans relies heavily on the cytokine IL-17A and its ability to stimulate a coordinated antifungal response by the multilayered oral epithelium. Aggor et al. used a mouse model of oral candidiasis to investigate the contribution of IL-22, another cytokine produced by many type 17 lymphocytes, to antifungal immunity. The rapid induction of IL-22 after oral Candida infection enhanced IL-22–dependent proliferation of the basal epithelial layer, thereby sustaining the ability of suprabasal epithelial cells expressing IL-17 receptors to respond to IL-17A with induction of immune effectors capable of repelling fungal infection. These findings provide new mechanistic insights into the cooperative antifungal effects of IL-22 and IL-17A in defending the host against oral candidiasis.


Oropharyngeal candidiasis (OPC; thrush) is an opportunistic infection caused by the commensal fungus Candida albicans. Interleukin-17 (IL-17) and IL-22 are cytokines produced by type 17 lymphocytes. Both cytokines mediate antifungal immunity yet activate quite distinct downstream signaling pathways. While much is now understood about how IL-17 promotes immunity in OPC, the activities of IL-22 are far less well delineated. We show that, despite having similar requirements for induction from type 17 cells, IL-22 and IL-17 function nonredundantly during OPC. We find that the IL-22 and IL-17 receptors are required in anatomically distinct locations within the oral mucosa; loss of IL-22RA1 or signal transducer and activator of transcription 3 (STAT3) in the oral basal epithelial layer (BEL) causes susceptibility to OPC, whereas IL-17RA is needed in the suprabasal epithelial layer (SEL). Transcriptional profiling of the tongue linked IL-22/STAT3 not only to oral epithelial cell proliferation and survival but also, unexpectedly, to driving an IL-17–specific gene signature. We show that IL-22 mediates regenerative signals on the BEL that replenish the IL-17RA–expressing SEL, thereby restoring the ability of the oral epithelium to respond to IL-17 and thus to mediate antifungal events. Consequently, IL-22 signaling in BEL “licenses” IL-17 signaling in the oral mucosa, revealing spatially distinct yet cooperative activities of IL-22 and IL-17 in oral candidiasis.


Fungal infections are a serious threat to public health, but our understanding of immunity to fungi lags behind that of other microbes (1). Even today, there are no licensed vaccines to any fungal microbes (2, 3). Oropharyngeal candidiasis (OPC; thrush) is an opportunistic infection of the oral mucosa caused by the commensal fungus Candida albicans. OPC occurs commonly in the settings of HIV/AIDS, head and neck cancer radiation treatment, immunosuppressive therapies, or the suboptimal immune responses in infants and the elderly (4, 5). OPC is also a characteristic infection of patients with gene mutations impairing the interleukin-17 (IL-17)/T helper 17 (TH17) pathway, such as STAT3, ACT1, IL17RA, or IL17RC, among others (69). Consistently, whereas immunocompetent wild-type (WT) mice are resistant to OPC, corticosteroid immunosuppression or loss-of-function mutations in the IL-17 receptor (Il17ra and Il17rc) or related genes (Act1, Il23, Il12b, or Rorc) result in high susceptibility to OPC (1014). However, peak oral fungal burdens in susceptible Il17ra−/− mice are still lower than in animals immunosuppressed with corticosteroids (12), indicating that signals in addition to IL-17 are needed for full protection in OPC.

Although IL-17 is the eponymous cytokine of TH17 cells and other “type 17” lymphocytes, IL-22 is also characteristic of these cells (15). Multiple studies indicate that IL-22, like IL-17, helps control oral candidiasis. For example, OPC occurs in patients with autoimmune polyendocrine syndrome type 1 (APS-1), a congenital autoimmune syndrome caused by mutations in AIRE and characterized by circulating autoantibodies that neutralize not only IL-17A and IL-17F but also IL-22 (1619). Mice with IL-22 impairments [via gene deficiency or antibody (Ab) neutralization] are susceptible to OPC (12, 20, 21), and reduced IL-22 expression is associated with human chronic mucocutaneous candidiasis (CMC) (8). Nonetheless, relatively little is known about the mechanisms of antifungal immunity mediated by IL-22 in this setting or in other oral diseases.

The oral mucosa provides a vital physical barrier to limit pathogen invasion, yet mechanisms of oral mucosal immunity remain surprisingly understudied, especially compared with other mucosal tissues such as the lung or gut. During OPC, oral epithelial cells (OECs) sense the transition of C. albicans from a benign yeast morphotype to a damaging, invasive hyphal state (22). This early recognition is mediated, in part, by epidermal growth factor receptor (EGFR) family receptors and also involves sensing of oral tissue damage induced by a secreted fungal toxin, candidalysin (2327). This OEC immunosurveillance response triggers production of IL-17 from lymphocytes (both innate and adaptive) via IL-1–dependent signals (2830). IL-17 then activates essential antifungal responses in OECs, including myeloid and lymphoid chemoattractants and antimicrobial peptides (AMPs), particularly β-defensin-3 (BD3) (5, 12, 3133).

The oral mucosa is a stratified nonkeratinizing tissue composed of distinct epithelial layers (34, 35). A proliferative basal epithelial layer (BEL) undergoes a program of differentiation that gives rise to the postmitotic suprabasal epithelial layer (SEL). This differentiation process maintains the tissue and restores barrier immunity after infection or injury (3537). Each layer is characterized by expression of specific pairs of cytokeratin filaments. Like most stratified epithelia, BEL expresses keratin 5 (K5) and K14. However, the SEL expresses K4 and K13, which have a more restricted expression pattern. IL-17R signaling activity is generally restricted to nonhematopoietic cells (38, 39), and we previously demonstrated that IL-17RA in K13+ SEL cells is essential for immunity to OPC (31). Similar to the IL-17R, the IL-22 receptor is expressed mainly in nonhematopoietic cells and is implicated in gastric C. albicans infections (40), yet remarkably little is known about IL-22 signaling and function in the oral mucosa.

Here, we show that IL-22 functions nonredundantly with IL-17 to limit fungal infection. Although expression kinetics are similar between these cytokines, there were unexpected differences in cellular sources, downstream target gene expression, and the nature and localization of the essential cytokine-responsive cells within the stratified oral epithelium. Transcriptomic analysis revealed that IL-22 signaling through signal transducer and activator of transcription 3 (STAT3) induces proliferation and survival of BEL cells. Moreover, IL-22–dependent signals are required for renewing the IL-17–responsive SEL. Hence, IL-22/STAT3 signaling “licenses” the oral IL-17 signaling response, despite acting in a distinct epithelial layer.


IL-22 is nonredundant with IL-17 in mediating protection against OPC

To compare the roles of IL-22 and IL-17 in OPC, we tracked the time course of Il22 and Il17a mRNA expression in the oral mucosa (tongue) of WT immunocompetent mice after sublingual C. albicans infection (41, 42). Il22 and Il17a transcripts were not detectable at baseline but were induced contemporaneously at ~16 to 24 hours after infection. Expression of both peaked at 48 hours and returned to undetectable levels by 96 hours (Fig. 1A). As previously observed (12), Il22−/− mice were susceptible to OPC, with fungal loads consistently averaging ~103 colony-forming units (CFU)/g of tongue tissue at 4 to 5 days after infection, whereas control WT mice fully cleared the infection by this time point (Fig. 1B). Fungal loads in Il22−/− mice were typically ~1/2 log lower than in mice with IL-17 signaling defects (Il17ra−/− or Act1−/−), also consistent with previous findings (Fig. 1B) (12, 14, 43).

Fig. 1 IL-22 protects against OPC nonredundantly with IL-17RA.

The indicated mice were sublingually inoculated with cotton ball–saturated phosphate-buffered saline (Sham) or C. albicans (OPC). Each symbol represents one mouse. (A) Total mRNA from tongue homogenates of infected WT mice was subjected to qPCR for Il22 and Il17a and normalized to Gapdh at each time point. Graphs show mean ± SEM. Data are pooled from four to nine mice per group. (B) Fungal burdens were determined by CFU enumeration on YPD/Amp agar at day 5 after infection. Graphs show geometric mean ± SD. Data were pooled from three independent experiments. Dashed line indicates limit of detection (~30 CFU/g). (C) Tongue homogenates were prepared on day 2 after infection. Left: Representative FACS plot showing percent of CD11b+Ly6G+ neutrophils (gated on live, CD45+ cells). Right: Data from three independent experiments. (D and E) Il17a and Il17f in total RNA from tongue on day 2 after infection were assessed by qPCR relative to Gapdh. Graphs show mean ± SEM relative to sham-infected WT mice. (F) Mice were injected intraperitoneally with anti–IL-22 or isotype control IgG (150 μg) on days −1, 0, 1, 2, and 3 relative to infection. CFU was assessed on day 4, pooled from two independent experiments. (G) Mice were treated with anti–IL-17A or isotype control (IgG2a) (200 μg) injected intraperitoneally on days −1, 1, and 3 relative to infection. CFU was assessed on day 5, pooled from three independent experiments. (H) Weight loss was assessed daily, shown relative to day 0 in mice from (F). Data were analyzed by ANOVA or Student’s t test, with Mann-Whitney U test for fungal load analysis.

We next evaluated the impact of IL-22 on antifungal events in OPC. In line with previous data, Il17ra−/− mice exhibited impaired neutrophil recruitment to the tongue after infection, measured at day 2 (12). Unexpectedly, Il22−/− mice showed increased oral neutrophil frequencies and numbers (Fig. 1C and fig. S1A), despite no differences in fungal load compared with Il17ra−/− mice at this time point (fig. S1B). Il17a and Il17f mRNA were elevated in Il22−/− mice, which may explain this observation (Fig. 1, D and E). Because mice are naïve to C. albicans, the IL-17 produced during a first encounter derives from innate immune cells (4347). We previously showed that antigen-independent CD4+T cell receptor αβ+ (TCRαβ+) cells that express IL-17 [“natural” TH17 cells (nTH17) (48)] are required for effective immunity to OPC (29, 44, 49). These nTH17 cells undergo proliferative expansion during the first 2 days of C. albicans infection in an IL-1R–dependent manner (28, 29, 44). In Il22−/− mice, the frequencies of proliferating (Ki67+) CD4+TCRαβ+ cells were comparable to WT mice after infection (fig. S1C), indicating that IL-22 does not regulate nTH17 proliferation.

In view of the many similar functions ascribed to IL-22 and IL-17 at mucosal surfaces (50), we asked whether IL-22 acts redundantly with IL-17 in OPC. Treatment with neutralizing Abs against IL-22 in Act1−/− mice [which are fully impaired for IL-17R signaling (51)] resulted in higher fungal burdens than isotype-treated controls (Fig. 1F). Similarly, blocking IL-17A in Il22−/− mice led to higher fungal burdens compared with isotype-treated controls (Fig. 1G). Weight loss was more pronounced in mice lacking IL-22 than in WT mice, but addition of anti–IL-17A Abs did not cause further weight loss (Fig. 1H). Thus, IL-22 and IL-17 act cooperatively but nonredundantly to control OPC.

Determinants of IL-22 production in acute OPC

Conventional adaptive TH17 responses to C. albicans are triggered by sensing of β-glucans in the fungal cell wall via Dectin-1/CARD9 signaling, which induces TH17-polarizing cytokines (IL-23 and IL-6) (5258). Here, we assessed the essential triggers of IL-22 in innate responses to OPC. As expected, Il23r−/− mice showed impaired oral Il22 expression following C. albicans infection (Fig. 2A), consistent with elevated fungal loads seen in Il23r−/− mice (12, 30). However, mice lacking Dectin-1 (Clec7a−/−) or CARD9 induced Il22 mRNA normally (Fig. 2A). In some settings, IL-22 is induced in TH17 cells by the aryl hydrocarbon receptor (AhR) or serum amyloid A (SAA) (59, 60). However, Ahr−/− mice did not exhibit an obvious deficit in Il22 induction and were resistant to OPC (fig. S2, A and B). Similarly, SAA1/2-deficient mice were resistant to OPC, and Il17a, Il17f, and Il22 were induced normally in the oral mucosa (fig. S2, C and D). Because these prototypical IL-22–inducing signals were dispensable for acute IL-22 induction, we next evaluated the role of candidalysin, a recently described trigger of anti-Candida immunity. This fungal cytolytic peptide is generated by proteolytic cleavage of the fungal Ece1 protein (encoded by ECE1). Candidalysin is produced only by hyphae and is needed for optimal expression of IL-17 and proliferation of nTH17 cells after oral C. albicans challenge (29, 61). WT mice were infected with C. albicans strains lacking either the full ECE1 gene (ece1Δ/Δ), or just the candidalysin peptide (ClysΔ/Δ), or an ECE1 re-complemented strain (Rev). Infection with ece1Δ/Δ or ClysΔ/Δ caused reduced Il22 mRNA expression compared with Rev. (Fig. 2B). IL-22 protein levels were similarly affected, assessed using IL22TdTomato reporter mice (fig. S2E) (62). Notably, these strains showed similar fungal burdens at this time point (fig. S2F). Thus, IL-23 and candidalysin, but not Dectin-1/CARD9, AhR, or SAA1/2, are required for innate IL-22 production during OPC.

Fig. 2 Determinants of IL-22 induction in acute OPC.

The indicated mice were subjected to OPC. Each symbol represents one mouse. (A) Tongues were harvested on day 2 after infection, and Il22 mRNA was assessed by qPCR, normalized to Gapdh. Graphs show mean ± SEM relative to sham-infected WT mice. (B) WT mice were infected with the indicated C. albicans strains. Il22 mRNA in tongue on day 2 after infection was assessed by qPCR, normalized to Gapdh. Data were pooled from two independent experiments. (C) BM from indicated donors was transferred into irradiated recipients. After 6 to 9 weeks, mice were subjected to OPC and fungal burdens were assessed on day 5 after infection. Data were pooled from two experiments. (D) On day 2 after infection, tongue homogenates from Il22TdTomato mice were stained for TCRβ and TCRγδ and gated on the live CD45+TdTomato+ population. Left: Representative FACS plots. Right: Pooled results from three independent experiments. Each symbol represents data from two pooled tongues. Data were analyzed by ANOVA or Student’s t test, with Mann-Whitney U test for fungal load analysis. (E) Comparisons of the relative percentages of IL-22+ cells (left) or IL-17+ cells [data from (29)] isolated from tongues of mice 2 days after infection and analyzed by flow cytometry.

We next delineated the oral cellular sources of IL-22 during OPC using bone marrow (BM) chimeras created from WT and Il22−/− mice. As expected, mice receiving Il22−/− BM regardless of host genotype developed OPC, indicating that IL-22 in hematopoietic cells was required for C. albicans clearance (Fig. 2C). We then analyzed tongue tissue from IL22TdTomato reporter mice at day 2 after infection, the time point when Il22 mRNA expression peaks (Fig. 1A). There were almost no detectable CD45+TdTomato+ cells before infection, indicating that IL-22 is not expressed in the oral mucosa at baseline. After infection, however, a substantial population of reporter-positive cells was present (Fig. 2D). Of these, γδ-T cells constituted the dominant TdTomato+ population (58%), followed by CD4+TCRαβ+ cells (26%). A population of CD4TCRαβ+ cells (8%) and of TCR-negative cells (8%) also expressed the reporter (Fig. 2D). This expression pattern contrasts to some extent with that of IL-17, based on previous studies using an IL-17A fate tracking reporter mouse (Fig. 2E) (29, 44, 63).

IL-22 signaling in the BEL is required to control OPC

IL-22 signals via the IL-10R2 and IL-22RA1 subunits, and the latter is shared among several cytokines (64). Il22ra1−/− mice showed a similar, albeit not statistically identical, susceptibility to OPC compared with Il22−/− mice (Fig. 3A); the difference could be indicative of a contribution of other IL-22R–dependent cytokines, although this possibility was not pursued. By 14 days after infection, most Il22ra1−/− mice had cleared the infection (fig. S3A), which contrasts with Il17ra−/− mice that were previously shown to maintain oral fungal loads as long as 17 days after infection (12, 31). To identify cell compartments requiring IL-22RA1 in OPC, we created reciprocal BM chimeras with WT and Il22ra1−/− mice. Regardless of BM source, WT recipients were resistant to OPC, whereas irradiated Il22ra1−/− recipients failed to clear C. albicans (Fig. 3B). Thus, IL-22RA1–mediated signaling in nonhematopoietic cells is required for effective antifungal immunity.

Fig. 3 IL-22 signaling in the oral BEL is required for protection against OPC.

The indicated mice were subjected to OPC. (A) Fungal burdens were assessed on day 5 after infection. Bars show geometric mean ± SD. Data were pooled from two independent experiments. (B) BM from indicated donors was transferred into irradiated recipients. After 6 to 9 weeks, mice were subjected to OPC and fungal burdens were assessed on day 5 after infection. Data were pooled from two experiments. (C) Frozen sections from WT tongues were costained with 4′,6-diamidino-2-phenylindole (DAPI) and Abs against K13, K14, or IL-22RA1. SEL and BEL are indicated. Images are representative of a minimum of three sections. Scale bars, 200 μm. (D) Top: Fungal burdens were assessed on day 5 after infection. Data are pooled from three independent experiments. Bottom: IF staining of tongues from the indicated mice were costained with DAPI and α-IL-22RA1 Abs. Scale bars, 200 μm. (E) Top: All mice except Il22−/− were administered tamoxifen for 5 days before OPC, and fungal burden was assessed on day 5 after infection. Bars show geometric mean ± SD. Bottom: Frozen sections from tongues from the indicated mice were costained with DAPI and α-IL-22RA1. Scale bars, 200 μm. Data were pooled from three independent experiments and analyzed by ANOVA with Mann-Whitney U test.

Oral epithelial tissue is characterized by morphologically distinct expression of cytokeratins. K13 is expressed in differentiating SEL cells in the postmitotic, terminally differentiated layer, which overlies the proliferative K14-expressing BEL (35). Consequently, K13+ epithelial cells make first contact with C. albicans during hyphal invasion and are highly subject to fungal-induced tissue damage. Moreover, this SEL layer is then sloughed and swallowed as part of the antimicrobial clearance response (34). The SEL is replenished by proliferation of the underlying basal K14+ cells, which have stem-like properties, but how this is controlled in OPC is unclear. Immunofluorescence (IF) staining indicated that IL-22RA1 was prominent in the K14+ BEL, with some staining in K13+ SEL and papillae (Fig. 3C). Isotype controls verified Ab specificity (fig. S3, B and C).

To define the relative contributions of IL-22RA1 in OEC subtypes, we crossed Il22ra1fl/fl mice to K13Cre or K14CreERT2 mice (31, 65). Conditional deletion of Il22ra1 in SEL and BEL was efficient, as verified by IF (Fig. 3, D and E). Il22ra1K13 mice were resistant to OPC (mean fungal load, ~19), indicating that IL-22 signaling in SEL is dispensable for fungal control (Fig. 3D). Remarkably, these results show that IL-22 signals in a spatially distinct manner from IL-17, because deletion of Il17ra in K13+ cells resulted in significant susceptibility to OPC (31). To delete IL-22RA1 in BEL, Il22ra1K14ERT2 mice and controls (Il22ra1fl/fl, Il22ra1fl/-K14ERT2, and Il22ra1K14ERT2) were administered tamoxifen for 5 days and infected with C. albicans. In contrast to Il22ra1K13 mice, loss of Il22ra1 in K14+ cells led to markedly increased susceptibility to OPC (fungal load, ~508), demonstrating that IL-22R signaling is commensurate with its expression profile in the BEL (Fig. 3E). Hepatic IL-22R is needed for pulmonary bacterial immunity (66), but mice with a liver-specific deletion of IL-22RA1 (Il22ra1Alb) were resistant to OPC (fig. S3D). Hence, these data show that IL-22RA1 signaling in the K14+BEL, but not the K13+SEL, is required for oral fungal control and, accordingly, that IL-17 and IL-22 function in different epithelial compartments.

IL-22 activates STAT3 in the BEL to sustain epithelial proliferation and antifungal immunity

To determine the mechanisms by which IL-22 promotes immunity during OPC and whether this differs from IL-17–driven responses, we evaluated RNA sequencing (RNASeq) profiles of total tongue mRNA from C. albicans–infected Il22−/−, II17ra−/−, and WT mice. In keeping with the observation that IL-17 and IL-22 act nonredundantly, there were distinct gene sets induced by IL-22 (368 genes) versus IL-17RA (931 genes). There were also many overlapping genes controlled by both cytokines (215 genes) (Fig. 4A). As expected (15), gene set enrichment analysis (GSEA) identified down-regulation of IL-6/STAT3 gene sets in Il22−/− mice compared with Il17ra−/− and WT mice, with a normalized enrichment score (NES) of 1.4 (P < 0.05) (Fig. 4B). Consistently, Ingenuity Pathway analysis identified STAT3 as a central upstream regulator that integrates the IL-22 and IL-17RA transcriptional networks (Fig. 4C). Within this network, STAT3 was connected to proliferation and apoptosis genes and to transcription factors that regulate inflammation such as nuclear factor κB (NF-κB)/inhibitor of NF-κB (IκBξ; Nfkbiz) and mitogen-activated protein kinase (MAPK)/AP-1 (Fig. 4C). In line with these bioinformatic predictions, IL-22 induced STAT3 phosphorylation and IκBξ expression in a human OEC line (fig. S4, A and B).

Fig. 4 STAT3 in OECs is required for protection against OPC.

(A) RNASeq was performed on whole tongue mRNA from WT, Il22−/−, and Il17ra−/− mice subjected to OPC and harvested on day 1 after infection. Venn diagram of differentially regulated or overlapping genes in infected Il22−/− and Il17ra−/− compared with WT mice. A total of 215 genes were regulated by both IL-22 and IL-17RA, whereas 368 genes are regulated only by IL-22, and 931 genes were regulated only by IL-17RA. (B) GSEA enrichment of predicted IL-6/STAT3 gene sets in Il17ra−/− and Il22−/− mice from (A). (C) Ingenuity Pathway Analysis of RNASeq data from (A), indicating that STAT3 is an upstream regulator integrating Il22- and Il17ra-driven transcriptional networks. (D) IF staining of tongue frozen sections with DAPI and anti–p-STAT3 (Tyr705) in WT, Il22−/−, and Il17ra−/− mice harvested 2 days after infection. Scale bars, 200 μm. (E) qPCR of Il22 in tongue mRNA from WT or Il17ra−/− mice at 2 days after infection normalized to Gapdh. (F) IF staining of DAPI, p-STAT3 (Tyr705), and K14 in WT or Il22ra1−/− mice at 2 days after infection. Scale bars, 200 μm. (G) The indicated mice were subjected to OPC, and fungal burden was quantified at day 5 after infection. Data are pooled from three experiments. (H) All mice except Il22−/− were administered tamoxifen for 5 days and subjected to OPC, and fungal burden was assessed on day 5 after infection. Bars show geometric mean ± SD. Data were pooled from three experiments. Data were analyzed by ANOVA with Mann-Whitney U test.

In vivo, STAT3 phosphorylation was evident in the BEL after C. albicans infection in WT mice, but markedly reduced in Il22−/− and Il22ra1−/− mice (Fig. 4, D and F). Unexpectedly, STAT3 phosphorylation was increased in Il17ra−/− BEL during OPC, possibly due to elevated Il22 expression in these mice (Fig. 4, D and E). These results show that, although numerous stimuli have potential to activate STAT3, the dominant STAT3 response during OPC is mediated by IL-22RA1–dependent cytokines. This finding also supports the idea that there may be defective IL-22–driven signaling in the pathogenesis of OPC associated with STAT3 mutations (e.g., Job’s syndrome). K14 staining intensity by IF was reduced in Il22ra1−/− mice compared with WT (Fig. 4F). This was commensurate with RNASeq data that showed reduced Krt14 mRNA in Il22−/− mice (Fig. 4A), a phenomenon also observed in skin (67). In addition, we confirmed that mice lacking STAT3 in K14+ but not K13+ cells were susceptible to OPC (Fig. 4, G and H, and fig. S4C). Together, these data demonstrate a central role of STAT3 in the oral epithelium that sustains antifungal immunity.

GSEA also revealed increased expression of mitotic spindle checkpoint genes and decreased expression of cell death–associated genes in WT versus Il22−/− mice (Fig. 5A), NES 1.4 (P < 0.01) and 1.5 (P < 0.003), respectively. Specifically, positive cell cycle regulatory genes (Stat3, Jun, and Sphk1) were reduced, and negative regulators of cell cycle (Cdkn1c, Ddit3, and Ets1) were elevated (Fig. 5B). Concordantly, Il22ra1−/− mice showed decreased IF staining of Ki67 in K14+ cells after C. albicans infection, indicating that IL-22 is a major driver of BEL proliferation (Fig. 5C). Consistently, there was a trend to decreased bromodeoxyuridine (BrdU) incorporation in Il22−/− K14+ BEL cells after oral C. albicans infection (fig. S5A). We next evaluated cell cycle progression in EpCAM+ epithelial cells from Il22−/− and WT tongues by BrdU and 7-aminoactinomycin D (7-AAD) staining. As shown, there were comparable proportions of cells in the Gap2/mitotic (G2-M) phase, fewer in the synthesis (S) phase, and more in the G0-G1 phase (Fig. 5D and fig. S5, A and B). GSEA also suggested enrichment of cell death–associated and DNA damage response genes (Fig. 5A and fig. S5C). Il22−/− mice had higher frequencies of active caspase 3+ and terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling (TUNEL+) epithelial (EpCAM+) cells after infection (Fig. 5, E to G). Hence, IL-22 promotes BEL proliferation during OPC while limiting accumulation of apoptotic cells.

Fig. 5 IL-22 promotes cell survival and proliferation during OPC.

RNASeq was performed on tongue mRNA from WT, Il22−/−, or Il17ra−/− mice, isolated 24 hours after infection. (A) GSEA of mitotic spindle checkpoint pathway and cell death pathway genes. NES is shown on the y axis. (B) Heat map of cell cycle pathway genes in global differential gene expression analysis (Partekflow) in WT or Il22−/− mice. (C) IF staining of DAPI, Ki67, and K14 in WT and Il22ra1−/− mice at 2 days after infection. Data are representative of images from two mice per group. Scale bars, 100 μm. (D) BrdU was administered 24 hours after infection, and tongues were harvested on day 2 after infection. Cell cycle/apoptotic status of CD45EpCAM+ cells was determined by BrdU and 7-AAD staining. Data show mean ± SEM. (E) Frequency of CD45EpCAM+ cells staining positive for active (cleaved) caspase-3 in tongue homogenates at day 2 after infection measured by flow cytometry. Left: Representative FACS plot. Right: Pooled data from four independent samples showing mean fluorescence intensity (MFI) of cleaved caspase 3 within the CD45EpCAM+ compartment. Data were analyzed by Student’s t test. (F) DAPI and TUNEL staining of tongue sections from the indicated mice at day 2 after infection. Images are representative of four mice per group. Scale bars, 200 μm. (G) Quantification of TUNEL+ cells from (F). Data were analyzed by ANOVA and post hoc Tukey’s test.

IL-22 restores IL-17R expression and signaling in SEL to sustain antifungal immunity in OPC

In stratified epithelia such as the oral mucosa, proliferating BEL regenerates the postmitotic SEL (35, 36). Consistently, genes implicated in tissue repair, keratinization, and epithelial differentiation were impaired in Il22−/− mice compared with WT (Fig. 6A). Although IL-17RA signaling in K13+ SEL cells is critical for immunity to OPC (31), the factors that restore the SEL after sloughing have not been well defined. In view of the impaired proliferation of BEL in Il22−/− mice, we hypothesized that IL-22 might indirectly affect IL-17 signaling in OPC by restoring the IL-17RA–expressing SEL. Transcriptomic data and quantitative polymerase chain reaction (qPCR) revealed that there was decreased Il17ra mRNA expression in Il22−/− tongue (Fig. 6, B and C). IF staining revealed loss of IL-17RA in the SEL of WT mice after C. albicans infection, which was even more pronounced in Il22−/− mice (Fig. 6D). Flow cytometry also demonstrated that IL-17RA cell surface expression was reduced in WT and Il22−/− CD45EpCAM+ OECs after C. albicans infection, although the difference between WT and Il22−/− mice was modest (Fig. 6E). Consistent with reduced IL-17RA in oral epithelium, canonical IL-17 target genes associated with immunity to C. albicans were down-regulated in Il22−/− mice during OPC, including the essential AMP BD3 (Defb3) (Fig. 6F). Collectively, these data support a model in which IL-22 signaling promotes BEL-intrinsic signals that mediate anti-Candida immunity. Accordingly, IL-22 licenses IL-17R signaling by renewing the SEL and thereby restoring the capacity of the tissue to respond to IL-17R, which is vital in controlling OPC (Fig. 6G).

Fig. 6 IL-22 licenses IL-17 signaling during OPC.

(A) Heat map of genes implicated in tissue repair, wound healing, keratinization, and epithelial differentiation. (B) Heat map of IL-17 signature genes in OPC. (C) qPCR of Il17ra expression normalized to Gapdh in tongue tissue from the indicated mice subjected to OPC and analyzed on day 2 after infection. Data show mean ± SEM relative to sham-infected mice. Data were analyzed by ANOVA or Student’s t test. (D) IF staining of IL-17RA and DAPI in the indicated mice on day 2 after infection. (E) IL-17RA expression in CD45EpCAM+ OECs in WT or Il22−/− mice during OPC. Top: Representative FACS histogram. Bottom: Pooled data from two independent experiments. Scale bars, 200 μm. (F) Expression of BD3 (Defb3) mRNA in tongue from WT or Il22−/− mice during OPC, normalized to Gapdh. Data were analyzed by ANOVA or Student’s t test. (G) Diagram of stratified oral epithelium during a first encounter with C. albicans. Fungal hyphae induce cellular damage and secrete the peptide candidalysin, which facilitates tissue invasion and activates innate IL-17– and IL-22–producing lymphocytes [see (29, 61)]. IL-17 was shown previously to act dominantly on K13+ cells of the SEL (31). In contrast, IL-22/STAT3 promotes proliferation of the K14+ BEL that serves to restore the IL-17R–expressing SEL, thus maintaining IL-17–induced antifungal signals such as β-defensins and neutrophil responses that are required to mediate clearance of C. albicans. Diagram created on


Although IL-22 and IL-17 are both produced by type 17 cells, these cytokines are distinct in structure, receptors, and downstream signaling pathways (50). Even so, they are usually viewed interchangeably in the context of mucosal immunity. The protective function of IL-22 in oral candidiasis has been recognized for some time, yet its mechanisms of action are incompletely understood. In non-oral manifestations of C. albicans infection or colonization (systemic, vaginal, dermal, or gastric), IL-22 is not necessarily protective, emphasizing that specific cytokine responses even to the same pathogen are influenced by tissue milieu (12, 40, 6871).

Although multiple families of patients with CMC have been identified with mutations in IL-17R pathway genes (7, 72, 73), thus far, no humans with genetic IL-22 deficiencies have been reported. Nonetheless, it does not necessarily follow that this cytokine is unimportant in humans, only that its contribution to host defense is likely to be more modest than that of IL-17, or possibly that IL-22 deficiency is not compatible with life for some other reason. The former would be analogous to the role of IL-17F. In mice, loss of IL-17F alone does not cause OPC, yet dual blockade of IL-17F and IL-17A significantly increases susceptibility (43, 51). In humans, a family with IL-17F mutations has been described with CMC, suggesting a role in mucosal candidiasis (73). AIRE-deficient humans have circulating neutralizing Abs against IL-17F, IL-22, and IL-17A, thought to help explain the CMC manifestations in these patients (9, 1618). In most mammals, though not mice, TH17 cells also express IL-26, a member of the same cytokine family as IL-22, and this cytokine may also be a target of biologic therapy (74); however, there is no evidence for autoantibodies against IL-26 in AIRE deficiency nor have IL-26–deficient humans been described (16).

The mechanisms by which IL-17 and IL-22 act in OPC are divergent (50). Whereas loss of IL-17 impairs neutrophil recruitment (12, 31), IL-22 deficiency led to increased neutrophil tissue infiltration, presumably because Il17a is concomitantly elevated. These opposing activities on the neutrophil response may help to restrain excessive inflammation. The capacity of IL-17 to drive oral neutrophil recruitment in OPC is not observed by all who use this OPC model (28, 75), possibly reflecting altered microbiota or other distinctions among animal facilities. Hence, the events controlling oral mucosal immunity are complex and dynamic.

The immunology of the oral cavity is less well understood than other mucosae (34, 37). In part, this is due to technical challenges associated with isolating cells from oral mucosal sites and the paucity of tools available to interrogate cell types within this tissue (76). We show here that IL-22 and IL-17 are produced by and function in distinct oral cell subtypes in the setting of OPC. Unlike humans, mice do not harbor C. albicans as a commensal organism, and hence, acute oral infection with C. albicans reflects an innate, not adaptive, immune response. In previous studies, IL-17 was shown to be produced by several innate lymphocyte subsets, including TCRαβ+ nTH17 cells, which express CD4, have a diverse TCR repertoire, and are activated in a non–antigen-specific manner (29, 44). IL-17 is also detected to a lesser extent in γδ-T cells and, in some reports, ILC3 cells (29, 43, 44). Upon a recall encounter to C. albicans, mice generate conventional, antigen-specific TH17 cells that additionally contribute to the IL-17–producing pool, where they augment immunity to C. albicans (46, 47, 77). C. albicans–specific TH17 cells are abundant in humans, because C. albicans is encountered very early in life. Moreover, C. albicans–specific TH17 cells recognize and provide cross-reactive protection against other fungal species, which is likely why maintaining C. albicans as a commensal is evolutionarily advantageous (7881).

We observed that γδ-T cells were the predominant oral source of IL-22 during OPC, followed by nTH17 cells and TCR-negative cell types. These results parallel observations made in skin upon C. albicans infection, where γδ-T cells are the major source of type 17 cytokines (52). Because the sources of these cytokines were identified using reporter mice that may under-report, defining the relative differences in sources of IL-17 and IL-22 is worth pursuing in more detail (62, 63). Nonetheless, the induction requirements for IL-22 during OPC are remarkably similar to that of IL-17; namely, IL-23 and the fungal peptide candidalysin are crucial, whereas classical fungal pattern recognition receptors (PRRs) such as Dectin-1, CARD9, or activators of conventional TH17 cells such as AhR or SAA1/2 are dispensable (29, 59, 60, 77). The finding that AhR was not essential to induce IL-22 or clear C. albicans from the mouth, although initially unexpected, is consistent with observations that AhR facilitates EGFR phosphorylation in OPC, a key step in fungal adhesion, endocytosis, and invasion in OECs (25, 27, 82, 83).

IL-17 and IL-22 are typically depicted signaling on the same cell types to mediate mucosal immunity, which is the case in nonstratified epithelia such as gut or lung (50, 84). Hence, we did not anticipate that IL-17 and IL-22 would act upon spatially distinct cell types in the oral mucosa. To show this, we made use of a mouse that expresses Cre under control of the murine Krt13 proximal promoter, one of the first tools allowing relatively specific deletion in the oral mucosa (31). The K13Cre mouse deletes conditionally in the SEL of the tongue, buccal mucosa, esophagus, and vaginal tract, with no detectable Cre activity in BEL of any tissue examined. Using this system, we found that IL-17RA acts predominantly within K13+ cells in the setting of OPC, with fungal burdens only slightly reduced compared with a full Il17ra−/− animal (31). In contrast, deletion of IL-22RA1 or STAT3 in K13+ SEL did not affect fungal clearance during OPC. GSEA of RNASeq data predicted a role for the IL-22RA1/STAT3 axis in tissue proliferation and repair during C. albicans infection. IL-22R signaling, presumably through STAT3, was vital for proliferation and survival of the K14+BEL, consequently replenishing the SEL. Thus, IL-22 indirectly permits essential IL-17–driven antifungal events to occur by restoring the postmitotic superficial layer where IL-17RA is expressed. This spatial stratification of IL-22R versus IL-17R enforces the specificity, diversification, and integration of cues that ensure oral epithelial integrity, restrain undue inflammation, and promote antifungal immunity.

The oral mucosa is among the most resilient epithelial surfaces (85). By virtue of their location, superficial K13+ OECs are the first to make contact with C. albicans. In its noninvasive yeast (commensal) form, C. albicans causes no damage to the SEL, which was recently shown to be due to the fact that this form of the fungus does not produce the pore-forming virulence factor candidalysin (61). Accordingly, there is insufficient expression of IL-1 or other damage-associated molecular patterns (DAMPs) that would activate innate lymphocytes to produce IL-17 or IL-22. This creates an environment where benign commensal C. albicans colonization is favored. However, in conditions that are conducive to hypha formation and invasion into tissue, a different scenario ensues. As part of the response to fungal invasion, the damaged SEL is sloughed and swallowed, which helps to clear C. albicans. The resulting epithelial cell damage also triggers production of IL-1 and IL-36, which promote IL-17 and IL-23 expression, respectively (2830, 86). IL-17R signaling in the SEL up-regulates chemokines that recruit neutrophils and β-defensins that exert direct antifungal activity (12, 31, 33, 87). Hyphal invasion thus establishes an inflammatory milieu that is initiated by candidalysin-induced SEL damage, potentiated by IL-17– and candidalysin-induced effectors such as IL-1 and IL-36, and ultimately resolved upon clearance of the pathogen (29, 88).

IL-22 acts in many epithelial surfaces. Events in the oral epithelia are reminiscent of skin, where K14+ stem cells resupply superficial epithelial cells to maintain barrier integrity (36, 89). The skin also has “memory” properties that accelerate tissue repair after future insults, although whether this occurs in the mouth is unknown (90). IL-22RA1+ epithelial cells in colonic epithelium maintain genome integrity and limit apoptotic cell accumulation during genotoxic stress (91). Similarly, IL-22 signaling in the BEL during OPC promotes replacement of damaged epithelial cells, prevents accumulation of inflammatory apoptotic cells, preserves genome integrity, and, as shown here, helps maintain IL-17–driven antifungal activities.

IL-22 maintains the intestinal epithelial barrier during intestinal colonization of C. albicans (40, 92). Unlike the mouth, IL-17 is not protective in gut but rather promotes a tissue-destructive inflammatory cycle in response to C. albicans colonization (93). IL-17 signaling in gut is generally more reparative than inflammatory (9498), which is thought to explain why anti–IL-17 biologic therapies failed in treatment trials of Crohn’s disease (99). Anti–IL-17 biologics are associated with low but statistically significant rates of OPC, although it is rare for patients to stop therapy for this reason (100). Anti–IL-22 Abs are under evaluation for skin pathologies such as atopic dermatitis (101), but rates of C. albicans infections have not been reported. Our data predict that combinations of anti–IL-17 and anti–IL-22 could result in more severe mucosal candidiasis infections than either therapy alone.

GSEA data show that STAT3 is an integrating hub between the IL-22 and IL-17 signaling networks. The pathways inferred from bioinformatics data (proliferation, cell cycle analysis, and apoptosis) are commensurate with the known roles of IL-22 and STAT3 in epithelial proliferation and repair (64). Unexpectedly, IL-22/STAT3 gene sets and phosphorylation of STAT3 were enriched in the Il17ra−/− mice, although expression of other STAT3-activating cytokines [e.g., IL-6 and granulocyte colony-stimulating factor (G-CSF)] was impaired in the absence of IL-17 signaling. This could be due to elevated Il22 expression in Il17ra−/− mice, but likely reflects overall perturbation of immune response networks inferred from GSEA that may involve STAT3. Susceptibility to CMC in Job’s syndrome [also known as Hyper-IgE Syndrome (HIES)] patients with STAT3 mutations is attributed to impaired STAT3-dependent TH17 differentiation (9). However, in acute OPC in mice, STAT3 in CD4+ T cells is not required to control fungal loads (44). Thus, STAT3 likely modulates antifungal immunity beyond the hematopoietic compartment. Supporting this, we found that patients with HIES exhibit impairments in salivary antifungal immunity, with reduced levels of salivary AMPs including β-defensins and histatins (102). STAT3 deficiency in lacrimal epithelial cells enhances apoptosis, causing a Sjögren’s syndrome–like phenotype (103). STAT3 also regulates metabolism in various settings to meet energy needs for cell proliferation, and STAT3-regulated functions in mitochondria are becoming increasingly appreciated (104106). Hence, the IL-22/STAT3 axis coordinates antimicrobial immunity in a variety of environments.

In summary, these results reveal a deeper understanding of the antimicrobial defense functions of OECs and a complex interplay between distinct cytokines of the type 17 axis. It is clear that cells within the oral epithelium not only are physical barriers but also are topographically structured sentinels that work in concert to dictate the outcome of oral C. albicans colonization, the most common fungal infection of humans (4, 107).


Study design

The objective of this study was to delineate functions of IL-22 in immunity to OPC. We used a mouse model of C. albicans infection combined with knockout mice and fungal strains to interrogate sources and activators of IL-22, genes induced in the oral mucosa, and cell-specific functions of this cytokine in vivo. Sample sizes were determined by power analyses from pilot or previously published data. Mice of both sexes were assigned randomly to experimental cohorts. Unless noted, experiments were done two to three independent times. Data from multiple experiments were pooled unless noted. Investigators were not blinded to groups. No data were excluded. Endpoints were selected on the basis of previous kinetic studies (1 to 2 days for gene expression or IF staining in OPC, and 4 to 5 days for fungal load enumeration).


IL-22TdTomato, Il22ra1fl/fl, K13Cre, Card9−/−, K14CreERT2, STAT3fl/fl, Saa1/2−/−, and Il23r−/− mice were described (31, 62, 66, 108, 109). Il17ra−/− mice were from Amgen, and Il22−/− mice were from Genentech. All mice are available by materials transfer agreement (MTA). Other mice were from The Jackson Laboratory or Taconic Farms. Mice were on the C57BL/6 background and housed under specific pathogen–free conditions. Experiments were performed on age-matched mice (6 to 10 weeks) of both sexes. BM chimeras were generated by irradiation (9 Gy) followed by intravenous injection of 106 femoral/tibial BM. Immune reconstitution after 6 to 9 weeks was verified by CD45.1/2 fluorescence-activated cell sorting (FACS) staining. Experiments were performed in accordance with protocols approved by the University of Pittsburgh Institutional Animal Care And Use Committee and National Institute of Allergy and Infectious Diseases (NIAID) and followed guidelines in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health (NIH).

OPC and fungal strains

OPC was induced by sublingual inoculation with C. albicans (strain CAF2-1 or SC5314)–saturated cotton balls for 75 min under anesthesia, as described (12, 41). Tongue homogenates were prepared on a GentleMACS homogenizer (Miltenyi Biotec) with C-tubes, and CFU was determined by serial dilution plating on YPD/Amp (yeast extract-peptone-dextrose)/(ampicillin) agar. Anti–IL-22 Abs (Genentech, clone 8E-11) or control immunoglobulin G1 (IgG1; Bio X Cell) (150 μg) was injected intraperitoneally on days −1, 0, 1, 2, and 3 relative to infection. Anti–IL-17A or IgG2a control Abs (200 μg) were from Janssen Research & Development LLC (110) and administered intraperitoneally on days −1, 1, and 3. Mutant C. albicans lacking the full Ece1 protein (ece1Δ/Δ), the candidalysin peptide (ece1Δ/Δ + ECE1Δ184–279, herein called ClysΔ/Δ), or a complemented strain (ece1Δ/Δ + ECE1, herein called Rev) were described (61).

Flow cytometry

Flow cytometry of tongue homogenates was performed as described (44). Tongues were digested with deoxyribonuclease (DNase) I (1 mg/ml; Roche) and collagenase IV (0.7 mg/ml) in Hank’s balanced salt solution at 37°C. Filtered cell suspensions were stained directly or separated by Percoll gradient centrifugation. Abs were from eBioscience, BD Biosciences, BioLegend, or Abcam. Proliferation was assessed using the Foxp3/Transcription Factor Buffer Kit (eBioscience) with Ki67-APC (allophycocyanin)/peridinin chlorophyll protein (PerCP) (BD Pharmingen). To assess apoptosis, CD45EpCAM+ cells were stained with caspase-3 apoptosis kit (BD Biosciences). For cell cycle analysis, mice were injected intraperitoneally with 1 mg of BrdU (Abcam, BrdU flow kit, BD Biosciences, #552598) on day 1 after infection and tongues were harvested 24 hours later. Data were acquired on an LSR Fortessa and analyzed with FlowJo (BD Biosciences).

qPCR, RNASeq, and GSEA

Total tongue RNA was extracted using RNeasy kits (Qiagen) after homogenization in a Gentle MACS Dissociator (Miltenyi Biotec). Real-time PCR (qPCR) was performed as described and normalized to Gapdh (29). Primers were from QuantiTect Primer Assays (Qiagen). For RNASeq, complementary DNA (cDNA) libraries were prepared from tongue RNA harvested day 1 after infection (Nextera XT Kit) and RNASeq was performed on the Illumina NextSeq 500 platform by the Health Sciences Sequencing Core at the University of Pittsburgh. Sequencing reads were annotated and aligned to the UCSC (University of California, Santa Cruz) mouse reference genome (mm10, GRCm38.75) using HISAT (111). HISAT alignment files were used to generate read counts for each gene, and determination of differential gene expression was performed using the DE-seq package from Bioconductor (112). Unbiased hierarchical clustering of differentially expressed genes with P < 0.05 was calculated using CLC Genomics Workbench and Partek software. Relative expression values in heat maps are TPM (transcripts per kilobase million) values per sample that have been divided by the average expression across all samples. Partekflow and GSEA from the Broad Institute were used to calculate enrichment of genes in each set. Additional bioinformatics assistance was from the University of Pittsburgh Center for Research Computing and Health Sciences Library.

Histology, IF, and immunocytochemistry

Cryosections were stained per the Cell Signaling IF protocol ( The following Abs were used: IL-22RA1 (R&D Systems, clone MAB42941), K13 and K14 (Abcam, EPR3671 and EPR17350; Invitrogen LL002), Ki67 and anti–p-STAT3 (Tyr705) (Cell Signaling, #9145 and #9129), and IL-17RA (Amgen, clone M751). TUNEL staining was performed with an Apoptosis Detection kit (Millipore). Images were acquired on an EVOS FL microscope (Life Technologies). TUNEL+ cells were enumerated from 10 random fields per slide.

Cell culture, cytokine stimulations, and immunoblotting

TR146 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM)–F12 with 15% fetal bovine serum and antibiotics as described (31). For immunoblotting, 3 × 105 to 5 × 105 cells were seeded in serum-free DMEM-F12 overnight before cytokine stimulation. Recombinant human IL-22 (PeproTech) was used at 100 ng/ml. Abs to STAT3 were from Cell Signaling (#12640), and Abs to actin were from Millipore (clone C4-EMD).


Data were analyzed on Prism (GraphPad). PCR data were analyzed by one-way analysis of variance (ANOVA), Student’s t test, and post hoc tests were used as indicated in figure legends. Fungal load data were presented as geometric mean ± SD and analyzed by ANOVA and Mann-Whitney U test. Each symbol represents one mouse unless indicated. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.


Fig. S1. Immune cell responses to OPC in Il22−/− mice.

Fig. S2. SAA and AhR are dispensable for Il22 induction and protection against OPC.

Fig. S3. IL-22RA1 expression and function in the oral mucosa.

Fig. S4. Activation of STAT3 and IκBξ in OECs.

Fig. S5. IL-22 promotes proliferation and cell cycle progression during OPC.

Table S1. Raw data file.


Acknowledgments: We thank B. Hube (Hans-Knöll Institute, Jena) for providing C. albicans mutant strains. We thank U. Siebenlist (NIH) for Act1−/− mice, Amgen for Il17ra−/− mice and anti–IL-17RA Abs, Genentech for Il22−/− mice and anti–IL-22 Abs, Janssen for anti–IL-17A Abs, X. Lin (MD Anderson) for Card9−/− mice, and F. De Beer (U. Kentucky) for Saa1/2/ mice. We thank S. Filler, H. Conti, P. Sundstrom, M. McGeachy, H. Mullin, C. Morse, N. Weathington, T. Hand, J. Alcorn, R. Binder, and A. Verma for helpful input and critical reading. Funding: The following NIH grants supported this work: S.L.G.: DE022550, DE023815, and AI107825; P.S.B.: DK104680; J.R.N.: DE022550; G.T.-N.: HL135476; D.H.K.: AR071720 and AR060744; J.K.K.: HL139930; V.M.B.: AI110820. Equipment grant 1S1OD011925 supported flow cytometry. J.R.N. was supported by the Wellcome Trust (214229_Z_18_Z) and the NIH Research at Guys and St. Thomas’s NHS Foundation Trust and the King’s College London Biomedical Research Centre (IS-BRC-1215-20006). This work was partially supported by the Division of Intramural Research of the NIAID. Author contributions (CRediT taxonomy): Conceptualization: F.E.Y.A., D.H.K., J.R.N., V.M.B., M.S.L., and S.L.G.; methodology: S.K.D., V.M.B., J.K.K., M.S.L., and S.L.G.; investigation: F.E.Y.A., T.J.B., G.T.-N., N.W., R.D.B., W.S., B.M.C., A.C.S., C.M., and V.M.B.; formal analysis: F.E.Y.A. and V.M.B.; writing (original draft): F.E.Y.A. and S.L.G.; writing (review and editing): F.E.Y.A., T.J.B., G.T.-N., N.W., D.H.K., J.R.N., S.K.D., P.S.B., V.M.B., J.K.K., M.S.L., and S.L.G.; visualization: F.E.Y.A., T.J.B., V.M.B., M.S.L., and S.L.G.; supervision: S.K.D., V.M.B., J.K.K., M.S.L., and S.L.G.; funding acquisition: D.H.K., J.R.N., P.S.B., V.M.B., J.K.K., M.S.L., and S.L.G. Competing interests: S.L.G. previously received a grant from Janssen to evaluate anti–IL-17A Ab function in OPC (110). Candidalysin has been patented by King’s College London, UK and Hans-Knöll Institut, Jena, DE (U.S. Patent No.: 9,969,796; EU Patent No.: 2984103). The other authors declare no competing interests. Data and materials availability: Act1−/− mice are available by MTA from NIH. Il17ra−/− mice and anti–IL-17RA Abs (clone M751) are available under an MTA with Amgen Corp. Anti–IL-17A Abs are available by MTA with Janssen Research & Development LLC.). IL-22TdTomato mice are available by MTA from the National Cancer Institute. Anti–IL-22 Abs and Il22−/− mice are available by MTA from Genentech. Card9−/− mice are available by MTA from the MD Anderson Cancer Center. Saa1/2/ mice are available by MTA from the University of Kentucky. All raw sequencing reads from the RNASeq experiments were submitted to the NCBI sequence read archive (SRA) under BioProject accession number PRJNA599123. All other data needed to evaluate the conclusions in the paper are present in the paper or the Supplementary Materials.

Stay Connected to Science Immunology

Navigate This Article