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Single-cell analysis of human B cell maturation predicts how antibody class switching shapes selection dynamics

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Science Immunology  12 Feb 2021:
Vol. 6, Issue 56, eabe6291
DOI: 10.1126/sciimmunol.abe6291

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A human B cell roadmap

B cells undergo affinity maturation and class switch recombination (CSR) within germinal centers (GCs) to facilitate protective antibody responses against pathogens. King et al. performed single-cell transcriptome and antibody repertoire profiling of human tonsils to examine how antibody-based selection affects B cell maturation within GCs. In addition to generating a detailed resource of transcriptional programs associated with antibody class, they identified a pre-GC B cell state that expressed unmutated IgM and IgD but was enriched for CSR-associated genes and germline transcripts. These results indicate that a subset of B cells may be primed for CSR before GC entry and highlight antibody repertoire dynamics during B cell fate decisions in secondary lymphoid organs.

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