You are currently viewing the abstract.
View Full TextLog in to view the full text
AAAS login provides access to Science for AAAS members, and access to other journals in the Science family to users who have purchased individual subscriptions.
More options
Download and print this article for your personal scholarly, research, and educational use.
Buy a single issue of Science for just $15 USD.
Chaperoned vaccine primes pulmonary TRMs
Lung-dwelling tissue-resident memory T cells (TRMs) protect the lower respiratory system after exposure to previously encountered viral pathogens. Using a previously established amphiphilic vaccine formulation that binds avidly to albumin, Rakhra et al. investigated whether coordinated delivery to the lung mucosa of peptide antigen adjuvanted with a CpG oligonucleotide improved the generation of vaccine antigen-specific CD8+ pulmonary TRMs in mice compared with subcutaneous delivery. Intratracheal vaccine administration of the amphiphilic vaccine resulted in a substantial increase in cytokine-producing CD8+ TRMs in the lung and enhanced T cell–mediated protection against both virus and tumor cell challenges. These findings lay the groundwork for further development of amphiphilic mucosal vaccines delivered via an aerosol for induction of robust TRM-based immunity to viruses that threaten the human respiratory health.
Abstract
Tissue-resident memory T cells (TRMs) can profoundly enhance mucosal immunity, but parameters governing TRM induction by vaccination remain poorly understood. Here, we describe an approach exploiting natural albumin transport across the airway epithelium to enhance mucosal TRM generation by vaccination. Pulmonary immunization with albumin-binding amphiphile conjugates of peptide antigens and CpG adjuvant (amph-vaccines) increased vaccine accumulation in the lung and mediastinal lymph nodes (MLNs). Amph-vaccines prolonged antigen presentation in MLNs over 2 weeks, leading to 25-fold increased lung-resident T cell responses over traditional immunization and enhanced protection from viral or tumor challenge. Mimicking such prolonged exposure through repeated administration of soluble vaccine revealed that persistence of both antigen and adjuvant was critical for optimal TRM induction, mediated through T cell priming in MLNs after prime, and directly in the lung tissue after boost. Thus, vaccine persistence strongly promotes TRM induction, and amph-conjugates may provide a practical approach to achieve such kinetics in mucosal vaccines.
- Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works
This is an article distributed under the terms of the Science Journals Default License.