Science Immunology

Supplementary Materials

Supplementary Material for:

VpreB serves as an invariant surrogate antigen for selecting immunoglobulin antigen-binding sites

Mohamed Khass, Tessa Blackburn, Peter D. Burrows, Mark R. Walter, Emidio Capriotti, Harry W. Schroeder Jr.*

*Corresponding author. Email: hschroeder{at}uabmc.edu

Published 14 July 2016, Sci. Immunol. 1, aaf6628 (2016)
DOI: 10.1126/sciimmunol.aaf6628

This PDF file includes:

  • Fig. S1. Comparison of critical residues in mouse and human SLC and LC.
  • Fig. S2. Percentage rank order of individual amino acids by position and by RF in the germline sequence of the BALB/c WT DH repertoire and in the D-altered Δ-DDFL and ΔD-DμFS mice.
  • Fig. S3. Detection of pre-B cells expressing the pre-BCR.
  • Fig. S4. Quantification of BrdU incorporation in developing B cells by flow cytometry.
  • Fig. S5. Distribution of developing B cells that have incorporated BrdU or remained quiescent in G0.
  • Fig. S6. Flow cytometric gates used to evaluate the cell cycle distribution of developing BM B cell subsets.
  • Fig. S7. DH RF usage among in-frame HCs cloned from living and apoptotic pre-B cells.
  • Fig. S8. Comparison of predicted structures of pre-BCRs from apoptotic C and living C′ cells.
  • Table S1. Statistical tests reported in the upper panel of Fig. 4C.
  • Table S2. Analysis of predicted hydrogen bond interactions in positions 101 to 103 for living and apoptotic cell fractions C and C′.
  • Table S3. Statistical tests reported in the bottom panel of Fig. 4C.
  • Table S4. Statistical tests reported in Fig. 3C.
  • Table S5. Statistical tests reported in Fig. 3A.
  • Table S6. Hydrogen bond interaction in the IgG Fab–antigen PDB structures in positions 101 to 103.
  • Table S7. Statistical tests reported in Fig. 5C.
  • Table S8. Hydrogen bond interaction in the IgG Fab–antigen PDB structures in positions 101 to 103* without flanking tyrosines.

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Other Supplementary Material for this manuscript includes the following:

  • File S1 (.pdf format). List of the CDR-H3 sequences from WT and ΔD-DμFS cells in fractions B and D used in this work.
  • File S2 (.pdf format). List of the VH and CDR-H3 sequences from apoptotic and living C and C′ fractions used in this work.
  • File S3 (.pdf format). Table of Fab-Ag crystal structures.
  • File S4 (.pdf format). Sequence of the VH regions and alignments used in the comparative modeling protocol.
  • File S5 Excel file containing tabulated data for Fig. 1 and fig. S5.
  • File S6 PDF file containing the results of the statistical analysis of the data for Fig. 1 and fig. S5.

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