Science Immunology

Supplementary Materials

Supplementary Material for:

In-depth tissue profiling using multiplexed immunohistochemical consecutive staining on single slide

Romain Remark, Taha Merghoub, Niels Grabe, Geert Litjens, Diane Damotte, Jedd D. Wolchok, Miriam Merad,* Sacha Gnjatic*

*Corresponding author. Email: miriam.merad{at} (M.M.); sacha.gnjatic{at} (S.G.)

Published 14 July 2016, Sci. Immunol. 1, aaf6925 (2016)
DOI: 10.1126/sciimmunol.aaf6925

This PDF file includes:

  • Fig. S1. Efficiency assessment of the blocking method.
  • Fig. S2. MICSSS does alter tissue integrity.
  • Fig. S3. MICSSS does not alter the signal intensity.
  • Fig. S4. Visualization of multiple antigens on single cells using MICSSS.
  • Fig. S5. Sequential CD3 staining using MICSSS.
  • Fig. S6. Prognostic value of CD1c-positive cells.
  • Fig. S7. Correlation between automatic and manual quantifications.
  • Table S1. Primary Abs used for IHC.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Sequential staining using MICSSS on melanoma after ipilimumab treatment.
  • Movie S2 (.mp4 format). Automated image solution for multiparameter analysis at the cellular level.

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