Science Immunology

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Supplementary Material for:

PTPN22 inhibition resets defective human central B cell tolerance

Jean-Nicolas Schickel, Marcel Kuhny, Alessia Baldo, Jason M. Bannock, Christopher Massad, Haowei Wang, Nathan Katz, Tyler Oe, Laurence Menard, Pauline Soulas-Sprauel, Till Strowig, Richard Flavell, Eric Meffre*

*Corresponding author. Email: eric.meffre{at}yale.edu

Published 22 July 2016, Sci. Immunol. 1, aaf7153 (2016)
DOI: 10.1126/sciimmunol.aaf7153

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  • Fig. S1. New emigrant B cells isolated from NSG mice engrafted with PTPN22C/T or T/T HSCs display normal IgH repertoire.
  • Fig. S2. Defective central B cell tolerance in humanized mouse engrafted with HSCs carrying PTPN22 T allele(s).
  • Fig. S3. Overexpression of PTPN22 variants in NSG mice.
  • Fig. S4. 620W PTPN22 overexpression interferes with the central B cell tolerance checkpoint.
  • Fig. S5. Frequencies of antinuclear new emigrant B cells in PTPN22 C/T or T/T NSG mice treated with the LTV-1 PTPN22 inhibitor.
  • Fig. S6. Inhibition of PTPN22 expression during B cell development resets central B cell tolerance.
  • Fig. S7. Representative gating strategies.
  • Fig. S8. Unprocessed immunoblot images.
  • Table S1. Fetal donor characteristics.
  • Table S2. Repertoire and reactivity of antibodies from new emigrant B cells of mouse #1.
  • Table S3. Repertoire and reactivity of antibodies from new emigrant B cells of mouse #2.
  • Table S4. Repertoire and reactivity of antibodies from new emigrant B cells of mouse #3.
  • Table S5. Repertoire and reactivity of antibodies from new emigrant B cells of mouse #4.
  • Table S6. Repertoire and reactivity of antibodies from new emigrant B cells of mouse #5.
  • Table S7. Repertoire and reactivity of antibodies from new emigrant B cells of mouse #6.
  • Table S8. Repertoire and reactivity of antibodies from new emigrant B cells of mouse #7.
  • Table S9. Repertoire and reactivity of antibodies from new emigrant B cells of mouse #8.
  • Table S10. Repertoire and reactivity of antibodies from new emigrant B cells of mouse #9.
  • Table S11. Repertoire and reactivity of antibodies from new emigrant B cells of mouse #10.
  • Table S12. Repertoire and reactivity of antibodies from GFP-negative new emigrant B cells of mouse #11.
  • Table S13. Repertoire and reactivity of antibodies from GFP-positive new emigrant B cells expressing 620W PTPN22 from mouse #11.
  • Table S14. Repertoire and reactivity of antibodies from GFP-negative new emigrant B cells of mouse #12.
  • Table S15. Repertoire and reactivity of antibodies from GFP-positive new emigrant B cells expressing 620W PTPN22 from mouse #12.
  • Table S16. Repertoire and reactivity of antibodies from GFP-negative new emigrant B cells of mouse #13.
  • Table S17. Repertoire and reactivity of antibodies from GFP-positive new emigrant B cells expressing 620W PTPN22 from mouse #13.
  • Table S18. Repertoire and reactivity of antibodies GFP-positive new emigrant B cells expressing R620 PTPN22 from mouse #14.
  • Table S19. Repertoire and reactivity of antibodies from GFP-positive new emigrant B cells expressing R620 PTPN22 from mouse #15.
  • Table S20. Repertoire and reactivity of antibodies from GFP-positive new emigrant B cells expressing 263Q PTPN22 from mouse #16.
  • Table S21. Repertoire and reactivity of antibodies from GFP-positive new emigrant B cells expressing 263Q PTPN22 from mouse #17.
  • Table S22. Repertoire and reactivity of antibodies from new emigrant B cells of mouse #18 treated with 0.75 mg of the LTV-1 PTPN22 inhibitor.
  • Table S23. Repertoire and reactivity of antibodies from new emigrant B cells of mouse #19 treated with 0.75 mg of the LTV-1 PTPN22 inhibitor.
  • Table S24. Repertoire and reactivity of antibodies from new emigrant B cells of mouse #20 treated with 0.15 mg of the LTV-1 PTPN22 inhibitor.
  • Table S25. Repertoire and reactivity of antibodies from new emigrant B cells of mouse #21 treated with 0.15 mg of the LTV-1 PTPN22 inhibitor.
  • Table S26. Repertoire and reactivity of antibodies from GFP-negative new emigrant B cells of mouse #21.
  • Table S27. Repertoire and reactivity of antibodies from GFP-positive new emigrant B cells expressing PTPN22 shRNA from mouse #21.
  • Table S28. Repertoire and reactivity of antibodies from GFP-negative new emigrant B cells of mouse #22.
  • Table S29. Repertoire and reactivity of antibodies from GFP-positive new emigrant B cells expressing PTPN22 shRNA from mouse #22.
  • Table S30. Repertoire and reactivity of antibodies from GFP-negative new emigrant B cells of mouse #23.
  • Table S31. Repertoire and reactivity of antibodies from GFP-positive new emigrant B cells expressing PTPN22 shRNA from mouse #23.

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