Science Immunology

Supplementary Materials

Supplementary Material for:

Tissue-specific emergence of regulatory and intraepithelial T cells from a clonal T cell precursor

Angelina M. Bilate,* Djenet Bousbaine, Luka Mesin, Marianna Agudelo, Justin Leube, Andreas Kratzert, Stephanie K. Dougan, Gabriel D. Victora, Hidde L. Ploegh*

*Corresponding author. Email: ambilate{at}wi.mit.edu (A.M.B.); ploegh{at}wi.mit.edu (H.L.P.)

Published 26 August 2016, Sci. Immunol. 1, eaaf7471 (2016)
DOI: 10.1126/sciimmunol.aaf7471

This PDF file includes:

  • Fig. S1. Generation of the pTreg TN mouse.
  • Fig. S2. pTreg TN precursors do not facilitate development of Tregs in the thymus.
  • Fig. S3. Age-dependent accumulation of CD4+CD8α+ T cells in the small intestine of pTreg TN/RKO mice.
  • Fig. S4. Naïve CD4+ TN T cells from pTreg TN/RKO expand and differentiate into pTregs and CD4IELs in immunodeficient hosts.
  • Fig. S5. CD4+ T cells from pTreg TN/RKO mice are MHCII-restricted.
  • Fig. S6. Antibiotic treatment depleted the microbiota.
  • Fig. S7. Intestinal bacterial antigens induce proliferation of CD4+ T cells from pTreg TN/RKO mice.
  • Fig. S8. CD4+ T cells from pTreg TN/RKO expand and convert into CD4IEL in lymphopenic hosts obtained from Taconic but not from Jackson Laboratory.
  • Fig. S9. Mice from the WIBR animal facility are colonized by SFB.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). RNA-seq data sets.
  • Table S2 (Microsoft Excel format). Metadata file for RNA-seq data sets.
  • Table S3 (Microsoft Excel format). Excel file containing tabulated data for Figs. 1 to 4 and 7.

Download Tables S1 to S3

Files in this Data Supplement: