Science Immunology

Supplementary Materials

Supplementary Material for:

Class I HLA haplotypes form two schools that educate NK cells in different ways

Amir Horowitz,* Zakia Djaoud, Neda Nemat-Gorgani, Jeroen Blokhuis, Hugo G. Hilton, Vivien Béziat, Karl-Johan Malmberg, Paul J. Norman, Lisbeth A. Guethlein,* Peter Parham

*Corresponding author. Email: ahorowitz{at} (A.H.); lisbeth.guethlein{at} (L.A.G.)

Published 9 September 2016, Sci. Immunol. 1, eaag1672 (2016)
DOI: 10.1126/sciimmunol.aag1672

This PDF file includes:

  • Fig. S1. Worldwide analysis of HLA-B–HLA-C haplotype frequencies.
  • Fig. S2. Primate MHC class I leader sequence–derived peptides.
  • Fig. S3. Evolution and variation at position −21 in the leader sequence of catarrhine primate MHC-B.
  • Fig. S4. Distribution of −21M and −21T among HLA-B and HLA-A allotype groups.
  • Fig. S5. Worldwide analysis of HLA-A–HLA-B haplotype and Bw4 epitope frequencies.
  • Fig. S6. HLA-A, HLA-B, HLA-C, and HLA-E genotypes for the panel of blood donors.
  • Fig. S7. HLA-E dimorphism at position 107 does not explain the expression differences between M/M, M/T, and T/T donors.
  • Fig. S8. Gating strategy for major cell lineages in PBMCs.
  • Fig. S9. Determining subset composition of IFN-γ+ NK cells by Boolean gating.
  • Fig. S10. Determining substantial influence of −21 HLA-B dimorphism on the NK cell repertoire of expressed inhibitory HLA class I receptors.
  • Fig. S11. Effects of NKG2C expression on frequency of NKG2A+ NK cells.
  • Table S1. Antibody panel used for mass cytometry.

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