Science Immunology

Supplementary Materials

Supplementary Material for:

Myosin light chains 9 and 12 are functional ligands for CD69 that regulate airway inflammation

Koji Hayashizaki, Motoko Y. Kimura, Koji Tokoyoda, Hiroyuki Hosokawa, Kenta Shinoda, Kiyoshi Hirahara, Tomomi Ichikawa, Atsushi Onodera, Asami Hanazawa, Chiaki Iwamura, Jungo Kakuta, Kenzo Muramoto, Shinichiro Motohashi, Damon J. Tumes, Tomohisa Iinuma, Heizaburo Yamamoto, Yuzuru Ikehara, Yoshitaka Okamoto, Toshinori Nakayama*

*Corresponding author. Email: tnakayama{at}faculty.chiba-u.jp

Published 16 September 2016, Sci. Immunol. 1, eaaf9154 (2016)
DOI: 10.1126/sciimmunol.aaf9154

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Identification of Myl9/12 as interacting proteins for CD69.
  • Fig. S2. Impacts of polyclonal anti-Myl9/12 antibodies on OVA-induced airway inflammation.
  • Fig. S3. Myl9/12 expression in the lung.
  • Fig. S4. Myl9/12 protein expression in platelets.
  • Fig. S5. Detection of CD69+ antigen-specific CD4 T cells in the lung vasculature during airway inflammation.
  • Fig. S6. Effects of anti-Myl9/12 and antihuman CD69 antibodies on human CD69-mediated allergic airway inflammation.
  • Fig. S7. Distribution of CD69+ T cells and Myl9/12 expression in polyps of ECRS patients.
  • Fig. S8. Unmodified images and isotype control staining in immunohistochemistry.
  • Legend for movie S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Myl9/12 form net-like structures in blood vessels of the inflamed lung.

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