Science Immunology

Supplementary Materials

Supplementary Material for:

Exploiting a host-commensal interaction to promote intestinal barrier function and enteric pathogen tolerance

Virginia A. Pedicord,* Ainsley A. K. Lockhart, Kavita J. Rangan, Jeffrey W. Craig, Jakob Loschko, Aneta Rogoz, Howard C. Hang,* Daniel Mucida*

*Corresponding author. Email: vpedicord{at}rockefeller.edu (V.A.P.); hhang{at}rockefeller.edu (H.C.H.); mucida{at}rockefeller.edu (D.M.)

Published 22 September 2016, Sci. Immunol. 1, eaai7732 (2016)
DOI: 10.1126/sciimmunol.aai7732

This PDF file includes:

  • Fig. S1. Individual weight loss and colonization of GF mice.
  • Fig. S2. Individual weight loss and colonization of antibiotic-treated mice.
  • Fig. S3. Individual pathogen burden in antibiotic-treated mice.
  • Fig. S4. E. faecium colonization does not affect expression of S. Typhimurium pathogenicity genes in vivo.
  • Fig. S5. E. faecium colonization does not alter intestinal immune cell subsets.
  • Fig. S6. Individual weight loss and colonization of streptomycin-treated mice.
  • Fig. S7. Individual pathogen burden in streptomycin-treated mice.
  • Fig. S8. E. faecium elicits up-regulation of AMPs.
  • Fig. S9. Individual weight loss and colonization of Reg3g−/− mice.
  • Fig. S10. Reg3g is required for E. faecium–mediated decreases in S. Typhimurium invasion.
  • Fig. S11. E. faecium does not significantly affect tight junction protein transcription.
  • Fig. S12. Tamoxifen induction of MyD88 excision in iVillΔMyd88 mice.
  • Fig. S13. Individual weight loss and colonization of iVillΔMyd88 mice.
  • Fig. S14. E. faecium–mediated protection is maintained in the absence of MyD88 signaling in myeloid cells.
  • Fig. S15. Individual weight loss and colonization of Nod2−/− mice.
  • Fig. S16. L. plantarum-sagA induces improved bacterial segregation and MUC2 distribution.
  • Fig. S17. Individual weight loss and colonization of mice with CDI.
  • Legends for movies S1 to S8

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). MUC2 distribution is decreased after antibiotic treatment.
  • Movie S2 (.mov format). MUC2 distribution is restored after E. faecium treatment.
  • Movie S3 (.mov format). MUC2 distribution is decreased after antibiotic treatment in Reg3g+/+ mice.
  • Movie S4 (.mov format). MUC2 distribution is decreased after antibiotic treatment in Reg3g−/− mice.
  • Movie S5 (.mov format). MUC2 distribution is restored after E. faecium treatment in Reg3g+/+ mice.
  • Movie S6 (.mov format). MUC2 distribution is not restored after E. faecium treatment in Reg3g−/− mice.
  • Movie S7 (.mov format). L. plantarum-vector does not restore MUC2 distribution.
  • Movie S8 (.mov format). L. plantarum-sagA is sufficient to restore MUC2 distribution.

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