Science Immunology

Supplementary Materials

Supplementary Material for:

Bidirectional intragraft alloreactivity drives the repopulation of human intestinal allografts and correlates with clinical outcome

Julien Zuber, Brittany Shonts, Sai-Ping Lau, Aleksandar Obradovic, Jianing Fu, Suxiao Yang, Marion Lambert, Shana Coley, Joshua Weiner, Joseph Thome, Susan DeWolf, Donna L. Farber, Yufeng Shen, Sophie Caillat-Zucman, Govind Bhagat, Adam Griesemer, Mercedes Martinez, Tomoaki Kato, Megan Sykes*

*Corresponding author. Email: megan.sykes{at}

Published 7 October 2016, Sci. Immunol. 1, eaah3732 (2016)
DOI: 10.1126/sciimmunol.eaah3732

This PDF file includes:

  • Fig. S1. Representative gating strategy for graft-resident lymphoid populations.
  • Fig. S2. Intragraft mixed chimerism long after intestinal transplantation.
  • Fig. S3. Recipient chimerism in LPL according to clinical outcome.
  • Fig. S4. Absolute CD3+ IEL counts at day 10 and month 3 after transplant.
  • Fig. S5. Phenotypic features of IEL and LPL populations obtained from deceased organ donors.
  • Fig. S6. Role of NKG2D/MICA interaction in rejection.
  • Fig. S7. Frequency of recipient and donor cells in isolated IELs for seven patients.
  • Fig. S8. Frequency and overlap of HvG and non–HvG-reactive clones in native and transplanted intestines.
  • Fig. S9. Trajectory of GvH and non-GvH clones over time.
  • Fig. S10. Origin of GvH clones detected in early biopsies from patient 10.
  • Fig. S11. Representative contour plots demonstrating recipient specificity of anti–HLA-B8 antibody in patient 10.
  • Table S1. HLA class I typing and anti-HLA allele antibodies used to distinguish donor from recipient cells in intestinal transplant recipients.
  • Table S2. Epidemiological and clinical characteristics of patients and deceased donors.
  • Table S3. Source data for Fig. 1E.
  • Table S4. Number of unique clones detected in each population/specimen, and proportion of biopsy-detected clones identifiable as recipient, donor, or neither.

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