Science Immunology

Supplementary Materials

Supplementary Material for:

Group B Streptococcus circumvents neutrophils and neutrophil extracellular traps during amniotic cavity invasion and preterm labor

Erica Boldenow, Claire Gendrin, Lisa Ngo, Craig Bierle, Jay Vornhagen, Michelle Coleman, Sean Merillat, Blair Armistead, Christopher Whidbey, Varchita Alishetti, Veronica Santana-Ufret, Jason Ogle, Michael Gough, Sengkeo Srinouanprachanh, James W. MacDonald, Theo K. Bammler, Aasthaa Bansal, H. Denny Liggitt, Lakshmi Rajagopal,* Kristina M. Adams Waldorf*

*Corresponding author. Email: adamsk{at}u.washington.edu (K.M.A.W.); lakshmi.rajagopal{at}seattlechildrens.org (L.R.)

Published 14 October 2016, Sci. Immunol. 1, eaah4576 (2016)
DOI: 10.1126/sciimmunol.eaah4576

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Uterine contractions, AF cytokines, prostaglandin, and bacterial CFU from choriodecidual inoculations of hyperpigmented GBSΔcovR in four chronically catheterized pregnant M. nemestrina.
  • Fig. S2. Uterine contractions, AF cytokines, prostaglandin, and bacterial CFU from choriodecidual inoculations of nonpigmented GBSΔcovRΔcylE in four chronically catheterized pregnant M. nemestrina.
  • Fig. S3. Choriodecidual inoculation of hyperpigmented GBSΔcovR in pregnant NHPs induced bacterial invasion of fetal tissues and increased levels of TNF-α.
  • Fig. S4. Heat map demonstrating differential gene expression in chorioamniotic membranes from pregnant NHPs.
  • Fig. S5. Changes in chorioamniotic gene expression.
  • Fig. S6. Fluorescence-activated cell sorting (FACS) of neutrophils purified from human blood.
  • Fig. S7. The hemolytic pigment toxin induces membrane permeability in neutrophils.
  • Fig. S8. The hemolytic pigment induces neutrophil cell death independent of pyroptosis or apoptosis.
  • Fig. S9. Hyperhemolytic GBS induce ROS but are resistant to neutrophil killing.
  • Fig. S10. Increased NETs in the chorioamnion of NHPs infected with hyperpigmented GBSΔcovR.
  • Fig. S11. FACS of neutrophils purified from human maternal and fetal (cord) blood.
  • Fig. S12. The hemolytic pigment toxin induces membrane permeability in maternal and fetal neutrophils.
  • Table S1. Detailed pregnancy outcomes by animal.
  • Table S2. Selected genes showing differential expression in NHP chorioamniotic membranes infected with GBS strains or saline.
  • Table S3. qPCR probes and primers.
  • References (6886)

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