Science Immunology

Supplementary Materials

Supplementary Material for:

Inflammatory monocytes hinder antiviral B cell responses

Stefano Sammicheli, Mirela Kuka, Pietro Di Lucia, Nereida Jiménez de Oya, Marco De Giovanni, Jessica Fioravanti, Claudia Cristofani, Carmela G. Maganuco, Benedict Fallet, Lucia Ganzer, Laura Sironi, Marta Mainetti, Renato Ostuni, Kevin Larimore, Philip D. Greenberg, Juan Carlos de la Torre, Luca G. Guidotti, Matteo Iannacone*

*Corresponding author. Email: iannacone.matteo{at}hsr.it

Published 21 October 2016, Sci. Immunol. 1, eaah6789 (2016)
DOI: 10.1126/sciimmunol.aah6789

This PDF file includes:

  • Methods
  • Fig. S1. Generation and characterization of LCMV-specific light-chain transgenic mice.
  • Fig. S2. KL25 B cells do not produce LCMV-neutralizing Abs in vivo upon LCMV infection, regardless of the number of cells transferred.
  • Fig. S3. VSV and LCMV replication in popliteal LNs upon footpad infection.
  • Fig. S4. Spatiotemporal dynamics of B cell activation upon VSV infection.
  • Fig. S5. Spatiotemporal dynamics of B cell activation upon LCMV infection.
  • Fig. S6. Phenotype, recruitment determinants, and effect on viral titers of CD11b+Ly6Chi monocytes.
  • Fig. S7. KL25 B cells do not produce LCMV-neutralizing Abs in vivo upon LCMV infection, regardless of the virus dose.
  • Fig. S8. Characterization of CD11b+Ly6Chi monocytes in CX3CR1GFP/+;CCR2RFP/+ reporter mice.
  • Fig. S9. Inflammatory monocytes restrict LCMV-specific B cell motility in the IFA.
  • Fig. S10. KL25 B cell phenotype upon adoptive transfer into LCMV-infected WT or CCR2−/− mice.
  • Fig. S11. Inflammatory monocytes are recruited only transiently to VSV-infected LNs and do not suppress B cell responses.
  • Fig. S12. T cells are not required for inflammatory monocyte–mediated B cell suppression.
  • Legends for movies S1 to S4
  • References (43, 44)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Excel file containing tabulated data for Figs. 1 to 4 and figs. S1 to S12.
  • Movie S1 (.mp4 format). WT, VI10YEN, and KL25 B cells show indistinguishable motility in uninfected mice.
  • Movie S2 (.mp4 format). Spatiotemporal dynamics of B cell activation upon footpad VSV infection.
  • Movie S3 (.mp4 format). Spatiotemporal dynamics of B cell activation upon footpad LCMV infection.
  • Movie S4 (.mp4 format). Inflammatory monocytes recruited to LCMV-infected LNs interact with Ag-specific B cells.

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