Science Immunology

Supplementary Materials

Supplementary Material for:

Interferon-driven deletion of antiviral B cells at the onset of chronic infection

Benedict Fallet, Kerstin Narr, Yusuf I. Ertuna, Melissa Remy, Rami Sommerstein, Karen Cornille, Mario Kreutzfeldt, Nicolas Page, Gert Zimmer, Florian Geier, Tobias Straub, Hanspeter Pircher, Kevin Larimore, Philip D. Greenberg, Doron Merkler, Daniel D. Pinschewer*

*Corresponding author. Email: daniel.pinschewer{at}unibas.ch

Published 21 October 2016, Sci. Immunol. 1, eaah6817 (2016)
DOI: 10.1126/sciimmunol.aah6817

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Characterization of KL25H and KL25HL mice, FACS gating strategy to analyze transferred KL25HL B cells, and kinetics of KL25HL cell depletion in rCl13 infection.
  • Fig. S2. FACS gating strategy to analyze subsets of KL25HL B cell progeny and survival of KL25HL B cells in neonatal rCl13 carriers.
  • Fig. S3. GL7 and ASC gating strategy, representative FACS plots, and design of experiments studying LCMV antigen-experienced B cells.
  • Fig. S4. Responses of KL25HL B cells to lower-affinity rCl13 variants and antigen-dependent depletion of VSVG-specific B cells in rCl13 infection.
  • Fig. S5. IFNAR blockade alters TF and terminal differentiation profiles of B cells in rCl13 infection.
  • Fig. S6. Effects of depletion antibodies on serum IFN-α, virus loads, myeloid cell populations, and KL25HL B cell recovery, and impact of genetic inflammatory monocyte deficiency on KL25HL B cell recovery.
  • Fig. S7. Impact of αGr-1 and αIFNAR on inflammatory gene expression profiles in the spleen and BM.
  • Fig. S8. Individual impact of iNOS, FasL, IL-1β, IL-4, IL-6, and IL-12 on KL25HL B cell decimation.
  • Table S1. Impact of IFNAR blockade and of αGr-1 depletion on inflammatory gene expression profiles in the spleen and BM.
  • Table S2. Impact of IFNAR blockade and of αGr-1 depletion of inflammatory chemokine and cytokine responses in serum.
  • References (4468)

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