Science Immunology

Supplementary Materials

Supplementary Material for:

Adenovirus serotype 5 vaccine vectors trigger IL-27–dependent inhibitory CD4+ T cell responses that impair CD8+ T cell function

Rafael A. Larocca, Nicholas M. Provine, Malika Aid, M. Justin Iampietro, Erica N. Borducchi, Alexander Badamchi-Zadeh, Peter Abbink, David Ng'ang'a, Christine A. Bricault, Eryn Blass, Pablo Penaloza-MacMaster, Kathryn E. Stephenson, Dan H. Barouch*

*Corresponding author. Email: dbarouch{at}bidmc.harvard.edu

Published 4 November 2016, Sci. Immunol. 1, eaaf7643 (2016)
DOI: 10.1126/sciimmunol.aaf7643

This PDF file includes:

  • Fig. S1. Longitudinal characterization of antigen-specific CD8+ T cells after Ad5 and Ad26 immunization.
  • Fig. S2. OT-I and OT-II cell sorting and sample clustering.
  • Fig. S3. Characterization of Ad5-induced IL-10+CD4+ T cells.
  • Fig. S4. Ad26-elicited CD4+ T cell phenotype is independent of CD46 expression in mice.
  • Fig. S5. CD4+ T cell dysfunctional phenotype induced by Ad5 is independent of antigen persistence or liver tropism.
  • Fig. S6. Absence of IL-12 signaling shows minor effects after Ad5 immunization.
  • Fig. S7. IL-27/IL-35KO mice or treatment with α-IL-27 mAb enhances CD4+ T cell functionality.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Genes differentially expressed in CD8+ T cells after Ad5 versus Ad26 immunization.
  • Table S2 (Microsoft Excel format). Genes differentially expressed in CD4+ T cells after Ad5 versus Ad26 immunization.
  • Table S3 (Microsoft Excel format). Modulated gene sets in CD8+ T cells after Ad5 versus Ad26 immunization using the GSEA C2 collection.
  • Table S4 (Microsoft Excel format). Modulated gene sets in CD4+ T cells after Ad5 versus Ad26 immunization using the GSEA C2 collection.
  • Table S5 (Microsoft Excel format). Modulated gene sets in CD8+ T cells after Ad5 versus Ad26 immunization using the GSEA C7 collection.
  • Table S6 (Microsoft Excel format). Modulated gene sets in CD4+ T cells after Ad5 versus Ad26 immunization using the GSEA C7 collection.
  • Table S7 (Microsoft Excel format). Excel file containing tabulated data for Figs. 1, 3, 4, 5, and 7.

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