Supplementary Material for:

IL-10 induces a STAT3-dependent autoregulatory loop in T_H2 cells that promotes Blimp-1 restriction of cell expansion via antagonism of STAT5 target genes

Amanda C. Poholek,* Dragana Jankovic, Alejandro V. Villarino, Franziska Petermann, Angela Hettinga, Dror S. Shouval, Scott B. Snapper, Susan M. Kaech, Stephen R. Brooks, Golnaz Vahedi, Alan Sher, Yuka Kanno, John J. O'Shea*

*Corresponding author. Email: poholeka{at}pitt.edu (A.C.P.); john.oshea{at}nih.gov (J.J.O.)

Published 11 November 2016, Sci. Immunol. 1, eaaf8612 (2016)
DOI: 10.1126/sciimmunol.aaf8612

This PDF file includes:

• Fig. S1. Expression of Blimp-1 in T cell subsets in vitro.
• Fig. S2. The IL-12–STAT4–T-bet axis controls Blimp-1 expression in T_H1 cells.
• Fig. S3. IL-10 regulation of T_H1 and T_H2 cells.
• Fig. S4. T_H2 differentiation is normal in the absence of STAT3.
• Fig. S5. The absence of IL-10 signaling on T cells does not affect T_H2 cell differentiation in vivo.
• Fig. S6. Transduction of Blimp-1 GFP-expressing retrovirus.
• Fig. S7. Model of IL-10–driven Blimp-1 expression via STAT3 in T_H2 cells to promote cell death.
• Fig. S8. Representative gating strategies.
• Table S1. Flow cytometry antibodies.
• Table S2. Gene lists for GSEA analysis.
• Table S3. ChIP-qPCR custom-designed SYBR primers.

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Other Supplementary Material for this manuscript includes the following:

• Table S4. Excel file containing source data for Figs. 1, 3, 4, and 5.