Science Immunology

Supplementary Materials

Supplementary Material for:

A TSLP-complement axis mediates neutrophil killing of methicillin-resistant Staphylococcus aureus

Erin E. West,* Rosanne Spolski, Majid Kazemian, Zu Xi Yu, Claudia Kemper, Warren J. Leonard*

*Corresponding author. Email: erin.west{at}nih.gov (E.E.W.); wjl.helix{at}nih.gov (W.J.L.)

Published 18 November 2016, Sci. Immunol. 1, eaaf8471 (2016)
DOI: 10.1126/sciimmunol.aaf8471

This PDF file includes:

  • Materials and Methods
  • Fig. S1. TSLP does not directly kill MRSA, and normal neutrophil-depleted blood can still reduce MRSA burden.
  • Fig. S2. TSLP requires TSLPR and acts on human neutrophils to increase control of MRSA.
  • Fig. S3. TSLP is TSLPR-dependent and enhances the killing of both MRSA and S. aureus in vivo.
  • Fig. S4. TSLP treatment increases killing of MRSA by human neutrophils in a PI3K- and MAPK/ERK-dependent manner.
  • Fig. S5. TSLP treatment of mouse or human neutrophils does not affect phagocytosis.
  • Fig. S6. ROS- and complement-dependent TSLP-enhanced neutrophil killing.
  • Table S1. TSLP does not alter the transcriptional profile of human neutrophils.
  • Reference (47)

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