Science Immunology

Supplementary Materials

Supplementary Material for:

Long-term maintenance of human naïve T cells through in situ homeostasis in lymphoid tissue sites

Joseph J. C. Thome, Boris Grinshpun, Brahma V. Kumar, Masaru Kubota, Yoshiaki Ohmura, Harvey Lerner, Gregory D. Sempowski, Yufeng Shen, Donna L. Farber*

*Corresponding author. Email: df2396{at}cumc.columbia.edu

Published 2 December 2016, Sci. Immunol. 1, eaah6506 (2016)
DOI: 10.1126/sciimmunol.aah6506

This PDF file includes:

  • Fig. S1. Flow cytometry gating strategy.
  • Fig. S2. Naïve T cell proliferative turnover in tissues.
  • Fig. S3. Compartmentalization of RTEs in tissues.
  • Fig. S4. CD31 expression by CD4+ T cells in donor tissues with age.
  • Fig. S5. IL-4 and IL-10 production by naïve and memory T cells in lymphoid sites.
  • Fig. S6. TCR diversity of naïve T cells in lymphoid sites.
  • Fig. S7. Clonal overlap of naïve and memory T cells between tissues of individual donors.
  • Fig. S8. Selection of productive naïve TCR sequences by filtering.
  • Table S1. Donor information and figure usage for this study.
  • Table S2. Descriptive statistics for naïve T cell frequencies in different tissue stratified by age groups.
  • Table S3. TREC values for naïve T cells in thymus and lymphoid tissue of individual donors.
  • Table S4. Source data for all cytokines measured in this study.
  • Table S5. Summary TCR sequencing data for all tissue naïve and TEM cells analyzed.
  • Table S6. Calculation of overlap detection power using replicate samples.
  • Table S7. Antibody panels used in this study.

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