Science Immunology

Supplementary Materials

Supplementary Material for:

The TCF1-Bcl6 axis counteracts type I interferon to repress exhaustion and maintain T cell stemness

Tuoqi Wu,* Yun Ji, E. Ashley Moseman, Haifeng C. Xu, Monica Manglani, Martha Kirby, Stacie M. Anderson, Robin Handon, Elizabeth Kenyon, Abdel Elkahloun, Weiwei Wu, Philipp A. Lang, Luca Gattinoni, Dorian B. McGavern, Pamela L. Schwartzberg*

*Corresponding author. Email: pams{at}nhgri.nih.gov (P.L.S.); tuoqi.wu{at}nih.gov (T.W.)

Published 9 December 2016, Sci. Immunol. 1, eaai8593 (2016)
DOI: 10.1126/sciimmunol.aai8593

This PDF file includes:

  • Materials and Methods
  • Fig. S1. TFH-like CD8 T cells generated after chronic LCMV clone 13 infection.
  • Fig. S2. TCF1highTim3low virus-specific CD8 T cells are less exhausted and persist better during chronic viral infection.
  • Fig. S3. TCF1 is required for the differentiation of Tim3low virus-specific CD8 T cells and long-term persistence of T cell responses.
  • Fig. S4. TCF1 is intrinsically required for the development of Tim3low virus-specific CD8 T cells and sustained T cell responses.
  • Fig. S5. Numbers of TCF1-overexpressing and control P14 cells on day 8 after infection.
  • Fig. S6. GSEA of microarray data from TCF1 KO and overexpression experiments.
  • Fig. S7. Type I IFN blockade enhanced the generation of TCF1highTim3low virus-specific CD8 T cells.
  • Legends for tables S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Differentially expressed genes between Tim3Blimp1 and Tim3+Blimp1+ CD8 T cells.
  • Table S2 (Microsoft Excel format). Differentially expressed genes between Tcf7 KO and WT CD8 T cells.
  • Table S3 (Microsoft Excel format). Differentially expressed genes between TCF1-overexpressing and MIG P14 cells.
  • Table S4 (Microsoft Excel format). Raw data and statistical analyses.

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