Science Immunology

Supplementary Materials

Supplementary Material for:

Rapid profiling of RSV antibody repertoires from the memory B cells of naturally infected adult donors

Morgan S. A. Gilman, Carlos A. Castellanos, Man Chen, Joan O. Ngwuta, Eileen Goodwin, Syed M. Moin, Vicente Mas, José A. Melero, Peter F. Wright, Barney S. Graham, Jason S. McLellan, Laura M. Walker*

*Corresponding author. Email: laura.walker{at}adimab.com

Published 9 December 2016, Sci. Immunol. 1, eaaj1879 (2016)
DOI: 10.1126/sciimmunol.aaj1879

This PDF file includes:

  • Fig. S1. Purification of preF and postF sorting probes.
  • Fig. S2. Representative gating strategy for RSV F–specific B cell sorting.
  • Fig. S3. Anti–RSV F antibodies lack polyreactivity.
  • Fig. S4. Generation and validation of preF patch panel mutants.
  • Fig. S5. Antibody binding affinities for preF and postF.
  • Fig. S6. Antigenic site V resides between the epitopes recognized by D25, MPE8, and motavizumab.
  • Fig. S7. Degree of SHM does not correlate with neutralization potency.
  • Fig. S8. Neutralization of RSV subtype B.
  • Fig. S9. Neutralizing activities of preF-specific, cross-reactive, and postF-specific antibodies.
  • Fig. S10. Relationship between subtype B neutralization and epitope.
  • Table S1. Antigenic sites targeted by prototypic RSV antibodies.
  • Table S2. Efficiency of binder rescue from B cell sorting.
  • Table S3. Competition profile of antibodies used in binning experiments.
  • Legends for data files S1 to S3
  • Reference (62)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Binding, neutralization, epitope assignment, limited sequence features, and GenBank accession codes for the isolated antibodies.
  • Data file S2 (Microsoft Excel format). Site V–directed antibodies show convergent sequence features.
  • Data file S3 (Microsoft Excel format). Antibody variable gene sequences of HMPV cross-neutralizing antibodies.

Download Data files S1 to S3

Files in this Data Supplement: