Science Immunology

Supplementary Materials

Supplementary Material for:

Human lymphoid organ dendritic cell identity is predominantly dictated by ontogeny, not tissue microenvironment

Gordon F. Heidkamp, Jil Sander, Christian H. K. Lehmann, Lukas Heger, Nathalie Eissing, Anna Baranska, Jennifer J. Lühr, Alana Hoffmann, Katharina C. Reimer, Anja Lux, Stephan Söder, Arndt Hartmann, Johannes Zenk, Thomas Ulas, Naomi McGovern, Christoph Alexiou, Bernd Spriewald, Andreas Mackensen, Gerold Schuler, Burkhard Schauf, Anja Forster, Roland Repp, Peter A. Fasching, Ariawan Purbojo, Robert Cesnjevar, Evelyn Ullrich, Florent Ginhoux, Andreas Schlitzer, Falk Nimmerjahn, Joachim L. Schultze,* Diana Dudziak*

*Corresponding author. Email: diana.dudziak{at} (D.D.); j.schultze{at} (J.L.S.)

Published 16 December 2016, Sci. Immunol. 1, eaai7677 (2016)
DOI: 10.1126/sciimmunol.aai7677

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Gating strategy for DC subpopulations and monocytes from different human lymphohematopoietic organs.
  • Fig. S2. Comparative transcriptomic analysis of DCs, monocytes, and T and B cells.
  • Fig. S3. Marker genes for human lymphohematopoietic DC subsets.
  • Fig. S4. Cell surface molecule expression of lymphohematopoietic tissue DC subpopulations and monocytes.
  • Fig. S5. Generation of coexpression networks.
  • Fig. S6. Overlaying expression information onto the TR network of human DC subpopulations.
  • Fig. S7. Comparative analyses of different human lymphohematopoietic and non-lymphohematopoietic tissue DC and monocyte transcriptional data sets.
  • Fig. S8. ImmuneSigDB signatures related to the major DC subpopulations in blood, spleen, and thymus.
  • Legends for tables S1 to S8
  • References (7680)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Frequencies of human lymphohematopoietic DC subpopulations and expression of common DC or monocyte markers.
  • Table S2 (Microsoft Excel format). Gating strategy for sorting DC subpopulations, monocytes, and T and B cells.
  • Table S3 (Microsoft Excel format). Signature and marker genes for human lymphohematopoietic DC subsets.
  • Table S4 (Microsoft Excel format). Marker genes for the different lymphohematopoietic DC subpopulations.
  • Table S5 (Microsoft Excel format). Seven clusters of coexpressed genes.
  • Table S6 (Microsoft Excel format). TRs of human lymphohematopoietic DC subpopulations.
  • Table S7 (Microsoft Excel format). Similarities between lymphohematopoietic and non-lymphohematopoietic DC subsets.
  • Table S8 (Microsoft Excel format). Ranked list of ImmuneSigDB signatures.

Download Tables S1 to S8

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