Science Immunology

Supplementary Materials

Supplementary Material for:

The human thymus perivascular space is a functional niche for viral-specific plasma cells

Sarah Nuñez, Carolina Moore, Baoshan Gao, Kortney Rogers, Yessia Hidalgo, Pedro J. del Nido, Susan Restaino, Yoshifumi Naka, Govind Bhagat, Joren C. Madsen, María Rosa Bono, Emmanuel Zorn*

*Corresponding author. Email: ez2184{at}cumc.columbia.edu

Published 23 December 2016, Sci. Immunol. 1, eaah4447 (2016)
DOI: 10.1126/sciimmunol.aah4447

This PDF file includes:

  • Fig. S1. Histological analysis of B cells in the aging thymus (extended data).
  • Fig. S2. Relative area of CD20+ B cells in the thymic medulla.
  • Fig. S3. Histological analysis of the cytokeratin network in the aging thymus.
  • Fig. S4. Localization of B cells in epithelial and nonepithelial areas of the thymus (extended data).
  • Fig. S5. Visualization of CD31+ endothelial cells in the thymic PVS.
  • Fig. S6. Gating strategy for analyzing thymic B cell subsets.
  • Fig. S7. Thymic B cells display higher expression of MHC-II compared with thymic dendritic cells.
  • Fig. S8. CD138+ PCs in the thymic PVS (extended data).
  • Fig. S9. sIgG+ PCs in the thymic PVS (extended data).
  • Fig. S10. Detection of eosinophils in the thymus (extended data).
  • Fig. S11. Antibody secretion in unstimulated PBMCs.
  • Fig. S12. CD27 expression in IRF4+ cells.
  • Fig. S13. CD138+ PC gating strategy.
  • Fig. S14. Thymocyte subsets in the aging thymus.
  • Fig. S15. Tissue immunofluorescence controls.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Donor information and detailed flow cytometry data.
  • Table S2 (Microsoft Excel format). Frequency of B cell subsets in adult thymus and PBMCs.
  • Table S3 (Microsoft Excel format). CD28 expression in B cells and PCs from thymus and PBMCs.
  • Table S4 (Microsoft Excel format). Expression of CD27, CD38, CD19, and IRF4 in unstimulated and stimulated thymic B cells.
  • Table S5 (Microsoft Excel format). Detailed ELISpot and FluoroSpot data for each donor.
  • Table S6 (Microsoft Excel format). Antigen-specific ELISpot.

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