Science Immunology

Supplementary Materials

Supplementary Material for:

Dynamics of influenza-induced lung-resident memory T cells underlie waning heterosubtypic immunity

Bram Sl├╝tter, Natalija Van Braeckel-Budimir, Georges Abboud, Steven M. Varga, Shahram Salek-Ardakani, John T. Harty*

*Corresponding author. Email: john-harty{at}

Published 6 January 2017, Sci. Immunol. 1, eaag2031 (2017)
DOI: 10.1126/sciimmunol.aag2031

This PDF file includes:

  • Materials and Methods
  • Fig. S1. IAV-induced lung TRM express low levels of Eomes.
  • Fig. S2. Vac-induced lung TRM are transient.
  • Fig. S3. In vivo CFSE labeling of the lung.
  • Fig. S4. LCMV Armstrong infection does not induce lung TRM.
  • Fig. S5. CFSE dilution in the lung is not a consequence of active cell proliferation.
  • Fig. S6. Bcl-2 expression in late lung memory P14 cells.
  • Fig. S7. Total numbers of CD69+ and CD103+ cells in the lung parenchyma do not change up to 6 days after IN CFSE labeling.
  • Fig. S8. Deletion of lung TRM CD8 T cells after systemic administration of anti- CD8 antibody.
  • Fig. S9. Levels of IL-33 and TNF in lungs at different stages after IAV infection.
  • Fig. S10. Early versus late IAV-induced memory CD8 T cells differentially express genes involved in lymphocyte migration.
  • Table S1. Gene set of leukocyte migration pathway differentially expressed in late versus early memory P14 cells.
  • Table S2. Selected list of genes from the leukocyte migration pathway.

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Other Supplementary Material for this manuscript includes the following:

  • Table S3 (Microsoft Excel format). Raw data sets and statistical analyses.

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