Science Immunology

Supplementary Materials

Supplementary Material for:

Low CD21 expression defines a population of recent germinal center graduates primed for plasma cell differentiation

Denise Lau,* Linda Yu-Ling Lan,* Sarah F. Andrews, Carole Henry, Karla Thatcher Rojas, Karlynn E. Neu, Min Huang, Yunping Huang, Brandon DeKosky, Anna-Karin E. Palm, Gregory C. Ippolito, George Georgiou, Patrick C. Wilson*

*Corresponding author. Email: wilsonp{at}uchicago.edu (P.C.W.); lindalan{at}uchicago.edu (L.Y.-L.L.); denilau17{at}gmail.com (D.L.)

Published 27 January 2017, Sci. Immunol. 2, eaai8153 (2017)
DOI: 10.1126/sciimmunol.aai8153

This PDF file includes:

  • Fig. S1. Validation of the HA binding FACS assay.
  • Fig. S2. CD21lo cells can be isolated from frozen PBMCs.
  • Fig. S3. Peripheral blood, CD21lo, and memory cells are diverse in their immunoglobulin gene usage.
  • Fig. S4. Phylogenetic trees of experimentally validated influenza binding clones.
  • Fig. S5. Mutational load in influenza-reactive clones.
  • Fig. S6. Differential gene expression between CD21lo and memory B cells.
  • Fig. S7. CD21lo cells have elevated protein levels of Fas independent of HA specificity.
  • Table S1. Specificity of influenza-reactive antibodies.

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