Science Immunology

Supplementary Materials

Supplementary Material for:

IL–22 controls iron-dependent nutritional immunity against systemic bacterial infections

Kei Sakamoto, Yun-Gi Kim, Hideki Hara, Nobuhiko Kamada, Gustavo Caballero-Flores, Emanuela Tolosano, Miguel P. Soares, José L. Puente, Naohiro Inohara, Gabriel Núñez*

*Corresponding author. Email: gabriel.nunez{at}

Published 3 February 2017, Sci. Immunol. 2, eaai8371 (2017)
DOI: 10.1126/sciimmunol.aai8371

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Increased pathogen loads in blood and organs of Il22-/- mice orally infected with C. rodentium.
  • Fig. S2. Lack of lethality by heat-killed C. rodentium.
  • Fig. S3. Induction of pathogen-specific IgM and IgG is not impaired in Il22-/- mice.
  • Fig. S4. ler expression of WT and espB mutant of C. rodentium in vivo.
  • Fig. S5. EspH is not required for systemic growth of C. rodentium during infection.
  • Fig. S6. ler-regulated factors are essential for C. rodentium?induced hemolysis and lethality in Il22-/-.
  • Fig. S7. IL-22 does not regulate free iron and unsaturated iron-binding capacity in plasma.
  • Fig. S8. Hpx and Hp expression are induced by IL-22 in the liver.
  • Fig. S9. HP does not affect hemoglobin-promoted C. rodentium growth in vitro.
  • References (38, 39)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Excel file containing tabulated data for Figs. 1 to 6 and fig. S1.

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