Science Immunology

Supplementary Materials

Supplementary Material for:

Hypoxia determines survival outcomes of bacterial infection through HIF-1α–dependent reprogramming of leukocyte metabolism

A. A. Roger Thompson, Rebecca S. Dickinson, Fiona Murphy, John P. Thomson, Helen M. Marriott, Adriana Tavares, Joseph Willson, Lynne Williams, Amy Lewis, Ananda Mirchandani, Patricia Dos Santos Coelho, Catherine Doherty, Eilise Ryan, Emily Watts, Nicholas M. Morton, Shareen Forbes, Roland H. Stimson, Abdul G. Hameed, Nadine Arnold, Julie A. Preston, Allan Lawrie, Veronica Finisguerra, Massimiliano Mazzone, Pranvera Sadiku, Jermaine Goveia, Federico Taverna, Peter Carmeliet, Simon J. Foster, Edwin R. Chilvers, Andrew S. Cowburn, David H. Dockrell, Randall S. Johnson, Richard R. Meehan, Moira K. B. Whyte, Sarah R. Walmsley*

*Corresponding author. Email: sarah.walmsley{at}ed.ac.uk

Published 10 February 2017, Sci. Immunol. 2, eaal2861 (2017)
DOI: 10.1126/sciimmunol.aal2861

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  • Materials and Methods
  • Fig. S1. Hypoxia induces hypothermia, sickness behavior, and cardiac dysfunction in mice infected subcutaneously with S. aureus.
  • Fig. S2. The phenotype observed in hypoxic infected C57BL/6 mice is reproduced by heat-killed SH1000 and by intraperitoneal LPS in an outbred mouse strain and after dawn or dusk infection.
  • Fig. S3. Local immune responses are not impaired by hypoxia.
  • Fig. S4. Mice display stress responses to hypoxia but no demonstrable systemic inflammatory or oxidative response.
  • Fig. S5. Mice display preserved gross organ function in hypoxia.
  • Fig. S6. No evidence of increased pulmonary edema or pulmonary immune infiltration in hypoxic animals infected with S. aureus.
  • Fig. S7. Hypoxic preconditioning alters glucose uptake into tissues, and protection is conveyed via the bone marrow.
  • Fig. S8. Hypoxic preconditioning profoundly changes the global transcriptome of circulating leukocytes.
  • Fig. S9. Hypoxic preconditioning alters the HIF-1α pathway and downstream targets in circulating leukocytes.
  • Fig. S10. Preserved bacterial load, cytokine responses, and preconditioning responses with myeloid-specific suppression of HIF-1α.

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