Science Immunology

Supplementary Materials

Supplementary Material for:

Epigenomics of human CD8 T cell differentiation and aging

David M. Moskowitz, David W. Zhang, Bin Hu, Sabine Le Saux, Rolando E. Yanes, Zhongde Ye, Jason D. Buenrostro, Cornelia M. Weyand, William J. Greenleaf,* Jörg J. Goronzy*

*Corresponding author. Email: jgoronzy{at}stanford.edu (J.J.G.)

Published 17 February 2017, Sci. Immunol. 2, eaag0192 (2017)
DOI: 10.1126/sciimmunol.aag0192

This PDF file includes:

  • Methods
  • Fig. S1. Isolation of CD8 T cell subsets by cell sorting.
  • Fig. S2. Relationship of differentially open peaks in CD8 T cell differentiation with gene pathways and expression.
  • Fig. S3. Correlation between differentiation-associated chromatin openness changes in young individuals and changes in old individuals.
  • Fig. S4. Robustness analyses of estimates of chromatin accessibility changes with age.
  • Fig. S5. Chromatin openness and gene expression patterns associated with aging resemble those of differentiation.
  • Fig. S6. Aging is associated with an erosion of accessibility at promoters.
  • Fig. S7. NRF1 binding activity maintains the young CD8 T cell epigenetic landscape.
  • Fig. S8. Distribution of per-peak estimates of proportions of young CM cells in old na?ve.
  • Table S1. TF binding motif enrichment. Reference (52)

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Data values for OCRs and mitochondrial mass.

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