Science Immunology

Supplementary Materials

Supplementary Material for:

Successive annual influenza vaccination induces a recurrent oligoclonotypic memory response in circulating T follicular helper cells

Ramin Sedaghat Herati,* Alexander Muselman, Laura Vella, Bertram Bengsch, Kaela Parkhouse, Daniel Del Alcazar, Jonathan Kotzin, Susan A. Doyle, Pablo Tebas, Scott E. Hensley, Laura F. Su, Kenneth E. Schmader, E. John Wherry*

*Corresponding author. Email: ramin.herati{at} (R.S.H.); wherry{at} (E.J.W.)

Published 17 February 2017, Sci. Immunol. 2, eaag2152 (2017)
DOI: 10.1126/sciimmunol.aag2152

This PDF file includes:

  • Fig. S1. ICOS+CD38+ cTFH express many markers of activation.
  • Fig. S2. ICOS+CD38+ cTFH are distinct from other subsets.
  • Fig. S3. Influenza vaccine–induced changes in cTFH subsets by flow cytometry.
  • Fig. S4. Influenza vaccine–induced changes in cTFH subsets by TCRseq.
  • Fig. S5. Longitudinal analysis of cTFH subsets by TCRseq.
  • Fig. S6. Comparison of tetramer clonotypes and AIM clonotypes with the cTFH clonotypic response.
  • Fig. S7. Intersubset relationships.
  • Table S1. Demographic data for clinical cohorts.
  • Table S2. Fluorochrome reagents and antibody clones used.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Raw data for Figs. 1 to 7 and figs. S1 to S7.

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