Science Immunology

Supplementary Materials

Supplementary Material for:

The checkpoint for agonist selection precedes conventional selection in human thymus

Greet Verstichel, David Vermijlen, Liesbet Martens, Glenn Goetgeluk, Margreet Brouwer, Nicolas Thiault, Yasmine Van Caeneghem, Stijn De Munter, Karin Weening, Sarah Bonte, Georges Leclercq, Tom Taghon, Tessa Kerre, Yvan Saeys, Jo Van Dorpe, Hilde Cheroutre, Bart Vandekerckhove*

*Corresponding author. Email: bart.vandekerckhove{at}ugent.be

Published 24 February 2017, Sci. Immunol. 2, eaah4232 (2017)
DOI: 10.1126/sciimmunol.aah4232

This PDF file includes:

  • Fig. S1. PD-1+ T cell subsets in the human thymus.
  • Fig. S2. Gating strategy for PD-1+ CD8αβ+ (CD8αα+) T cells in the human thymus.
  • Fig. S3. Gating strategy for PD-1+ CD8αβ+ (CD8αα+) T cells in human cord blood.
  • Fig. S4. CD8αα+ CD8αβ+ T cells in human peripheral blood in infants.
  • Fig. S5. CD8αα+ CD8αβ+ T cells in human small intestinal epithelium and liver.
  • Fig. S6. NELL2 expression on tissue and circulating T cell subsets.
  • Fig. S7. Cytokine-induced proliferation of CD8αα+ T cells.
  • Fig. S8. Differential survival upon TCR activation for CD8αα and CD8αα+ T cells.
  • Fig. S9. TP blast express low levels of hallmark DP genes.
  • Fig. S10. Survival of post–β-selection subsets in vitro.
  • Fig. S11. In vitro differentiation of post–β-selection subsets in the absence of stimulation.
  • Fig. S12. Proliferation of post–β-selection subsets in vitro.
  • Fig. S13. TRAV gene usage in thymic CD8αα+ and CD8αα T cells.
  • Fig. S14. TRAV gene usage in cord blood CD8αα+ and CD8αα T cells.
  • Table S1. CD8αβ+ T cell fractions in the human thymus. immunology.sciencemag.org/cgi/content/full/2/8/eaah4232/DC1
  • Table S2. CD8αβ+ T cell fractions in human cord blood.
  • Table S3. CD8αα+ T cell–associated gene sets.
  • Table S4. CD8αα+ T cells and TP blast precursors show enrichment for early TRAV and TRAJ genes.
  • Table S5. Primer sequences
  • Table S6. Gene sets used for GSEA analyses

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