Science Immunology

Supplementary Materials

Supplementary Material for:

PPAR-γ promotes type 2 immune responses in allergy and nematode infection

Ting Chen, Christopher A. Tibbitt, Xiaogang Feng, Julian M. Stark, Leona Rohrbeck, Lisa Rausch, Saikiran K. Sedimbi, Mikael C. I. Karlsson, Bart N. Lambrecht, Gunilla B. Karlsson Hedestam, Rudi W. Hendriks, Benedict J. Chambers, Susanne Nyl?n, Jonathan M. Coquet*

*Corresponding author. Email: Jonathan.Coquet{at}ki.se

Published 10 March 2017, Sci. Immunol. 2, eaal5196 (2017)
DOI: 10.1126/sciimmunol.aal5196

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  • Materials and Methods
  • Fig. S1. Normal expression of IL-4 and IL-17 by lung memory CD4 T cells after HDM sensitization and challenge.
  • Fig. S2. Representative profiles of mesLN cells after H. polygyrus and graphs of cytokine production in Peyer?s patches.
  • Fig. S3. The effects of PPAR-γ on IL-5, IL-13, and ST2 expression are cellintrinsic.
  • Fig. S4. Normal BAL eosinophilia and ST2 expression after HDM administration into control Lck-Cre+ WT mice.
  • Fig. S5. Sort strategy for whole-genome expression arrays.
  • Fig. S6. ILC2 in VAT express PPAR-γ but are not targeted for Pparg gene deletion in PpargLckΔ mice.
  • Fig. S7. The effect of PPAR-γ on ILC2 frequency after HDM administration and H. polygyrus infection.
  • Fig. S8. Normal ILC2 responses in PpargLckΔ mice. Fig. S9. Normal frequencies of ST2+ Tregs at baseline in various organs.
  • Fig. S10. Analyzing the requirement for PPAR-γ in Tregs in the HDM and H. polygyrus model.

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