Science Immunology

Supplementary Materials

Supplementary Material for:

Up-regulation of LFA-1 allows liver-resident memory T cells to patrol and remain in the hepatic sinusoids

H. A. McNamara, Y. Cai, M. V. Wagle, Y. Sontani, C. M. Roots, L. A. Miosge, J. H. O'Connor, H. J. Sutton, V. V. Ganusov, W. R. Heath, P. Bertolino, C. G. Goodnow, I. A. Parish, A. Enders, I. A. Cockburn*

*Corresponding author. Email: ian.cockburn{at}

Published 17 March 2017, Sci. Immunol. 2, eaaj1996 (2017)
DOI: 10.1126/sciimmunol.aaj1996

This PDF file includes:

  • Materials and Methods
  • Fig. S1. CD44, VCAM-1, and β2-microglobulin are not required for CD8+ T cell motility in the liver.
  • Fig. S2. Phenotype of ITGAL-C77F mice.
  • Fig. S3. Similar phenotypes of WT and Itgal-/- cells after in vitro priming.
  • Fig. S4. Expression of CD11a on different populations of OT-I T cells.
  • Fig. S5. CD11ahi cells in the liver and lung are CD69+, KLRG1-, CD103-, and IVAb+ after sporozoite immunization.
  • Fig. S6. LCMV infection induces populations of CD11hi cells in the liver and lung that are phenotypically similar to those seen after sporozoite immunization.
  • Fig. S7. NKT cells have a similar CD11ahi CD69+ KLRG1- phenotype to liver TRM cells.
  • Fig S8. LFA-1 is required for the retention of cells in the liver and lung in the steady state.
  • Fig. S9. LFA-1 is required for the formation of TRM cells in the liver in the steady state.
  • Legends for movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Anti–ICAM-1 inhibits effector T cell migration in the hepatic sinusoids.
  • Movie S2 (.mov format). LFA-1–deficient cells display impaired motility in the liver.
  • Movie S3 (.mov format). Migration of OT-I cells in the livers of mice 1 week after sporozoite immunization.
  • Movie S4 (.mov format). Migration of OT-I cells in the livers of mice 4 weeks after sporozoite immunization.

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