Science Immunology

Supplementary Materials

Supplementary Material for:

Host sirtuin 1 regulates mycobacterial immunopathogenesis and represents a therapeutic target against tuberculosis

Catherine Y. Cheng, Nuria M. Gutierrez, Mardiana B. Marzuki, Xiaohua Lu, Taylor W. Foreman, Bhairav Paleja, Bernett Lee, Akhila Balachander, Jinmiao Chen, Liana Tsenova, Natalia Kurepina, Karen W. W. Teng, Kim West, Smriti Mehra, Francesca Zolezzi, Michael Poidinger, Barry Kreiswirth, Deepak Kaushal, Hardy Kornfeld, Evan W. Newell, Amit Singhal*

*Corresponding author. Email: amit_singhal{at}immunol.a-star.edu.sg

Published 24 March 2017, Sci. Immunol. 2, eaaj1789 (2017)
DOI: 10.1126/sciimmunol.aaj1789

This PDF file includes:

  • Methods
  • Fig. S1. SIRT1 expression in Mtb-infected human cells and in the peripheral blood of humans infected with Mtb and other diseases.
  • Fig. S2. SIRT1 is expressed by CD68/CD163 macrophages in granulomas of Mtbinfected macaques.
  • Fig. S3. Down-regulation of SIRT1 expression by mycobacteria and control of mycobacterial growth by RES and SRT.
  • Fig. S4. RES modulates the global gene expression during Mtb infection.
  • Fig. S5. SIRT1 activation induces LC3 expression and phagosome-lysosome fusion in mycobacteria-infected THP-1 cells.
  • Fig. S6. SRT reduces tissue Mtb load and Mtb-derived lung pathology, and SIRT1 deficient mouse has enhanced inflammatory response.
  • Fig. S7. Examples of staining for each antibody used for the mass cytometry analysis.
  • Fig. S8. Heat plot summary of average median expression of each cellular marker analyzed for the 28 clusters identified and rough descriptions of each cluster.
  • Fig. S9. Manual gating strategy used to analyze mass cytometry and flow cytometry data.
  • Fig. S10. Validation of tSNE-guided lung populations.
  • Fig. S11. Lung myeloid cell population changes upon Mtb infection and SRT treatment.
  • Fig. S12. Uncropped images of the Western blots.
  • Table S1. TB data sets used in this study.
  • Table S3. Enrichment of significant GOs in 3062 differentially regulated genes between RES-treated, Mtb-infected THP-1 cells (R) and untreated, Mtb-infected THP-1 cells (I).
  • Table S5. Antibodies used for mass cytometry analysis listing metal conjugate, antibody clone name, and supplier of each marker.
  • Table S6. Sequences of primers used in this study.
  • Table S7. P values for Fig. 1.
  • Table S8. P values for Fig. 2.
  • Table S9. P values for Fig. 3.
  • Table S10. P values for Fig. 4.
  • Table S11. P values for Fig. 5.
  • Table S12. P values for Fig. 6B.
  • Table S13. P values for Fig. 6 (C to H).

Download PDF

Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Differentially expressed genes between Mtb-infected THP-1 cells treated with RES and untreated cells.
  • Table S4 (Microsoft Excel format). Enrichment of significant canonical pathways (IPA analysis) in differentially regulated genes between RES-treated, Mtb-infected THP-1 cells (R) and untreated, Mtb-infected THP-1 cells (I).

Files in this Data Supplement: