Science Immunology

Supplementary Materials

Supplementary Material for:

LAG3 limits regulatory T cell proliferation and function in autoimmune diabetes

Qianxia Zhang, Maria Chikina, Andrea L. Szymczak-Workman, William Horne, Jay K. Kolls, Kate M. Vignali, Daniel Normolle, Maria Bettini, Creg J. Workman, Dario A. A. Vignali*

*Corresponding author. Email: dvignali{at}pitt.edu

Published 31 March 2017, Sci. Immunol. 2, eaah4569 (2017)
DOI: 10.1126/sciimmunol.aah4569

This PDF file includes:

  • Fig. S1. Up-regulation of LAG3 expression on islet-infiltrating lymphocytes.
  • Fig. S2. Generation of conditional Lag3-knockout mice.
  • Fig. S3. Reduced lymphocyte infiltration into islets in the absence of LAG3 on Tregs.
  • Fig. S4. Intrinsic and extrinsic impact of Treg-expressed LAG3 on T cell proliferation.
  • Fig. S5. Intrinsic and extrinsic impact of Treg-expressed LAG3 on Bcl2 expression.
  • Fig. S6. Effector cytokine production in the absence of LAG3 on Tregs.
  • Fig. S7. Phenotypic and functional analyses on Lag3-deficient Tregs.
  • Fig. S8. Treg signature genes are affected by islet microenvironment.
  • Fig. S9. Consistency between independent replicates of RNA-seq.
  • Fig. S10. A group of genes are down-regulated in intra-islet WT Tregs but are still maintained in Lag3-deficient Tregs.
  • Fig. S11. Lag3-deficient Tregs outcompeted WT Tregs in the same hosts.
  • Fig. S12. Eos expression and knockdown in Tregs.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Pathways differentially regulated in intra-islet Lag3-deficient versus WT Tregs.
  • Table S2 (Microsoft Excel format). Raw data sets.

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