Science Immunology

Supplementary Materials

Supplementary Material for:

Eomesodermin promotes the development of type 1 regulatory T (TR1) cells

Ping Zhang,* Jason S. Lee, Kate H. Gartlan, Iona S. Schuster, Iain Comerford, Antiopi Varelias, Md Ashik Ullah, Slavica Vuckovic, Motoko Koyama, Rachel D. Kuns, Kelly R. Locke, Kirrilee J. Beckett, Stuart D. Olver, Luke D. Samson, Marcela Montes de Oca, Fabian de Labastida Rivera, Andrew D. Clouston, Gabrielle T. Belz, Bruce R. Blazar, Kelli P. MacDonald, Shaun R. McColl, Ranjeny Thomas, Christian R. Engwerda, Mariapia A. Degli-Esposti, Axel Kallies, Siok-Keen Tey, Geoffrey R. Hill*

*Corresponding authors. Email: ping.zhang{at} (P.Z.); geoff.hill{at} (G.R.H.)

Published 7 April 2017, Sci. Immunol. 2, eaah7152 (2017)
DOI: 10.1126/sciimmunol.aah7152

This PDF file includes:

  • Materials and Methods
  • Fig. S1. TR1 cells are suppressive in vitro.
  • Fig. S2. TR1 cells display a distinct profile of markers.
  • Fig. S3. Eomes is required for the development of TR1 cells after BMT.
  • Fig. S4. Expression of Eomes during in vitro culture.
  • Fig. S5. Role of Eomes in the generation of TR1 cells in vitro.
  • Fig. S6. Eomes+ TR1 cells are dependent on Blimp-1, IL-27, and IL-10.
  • Fig. S7. Treg cell development in Il27r-/- and Il10-/- T cells after BMT.
  • Fig. S8. Both T-bet and Eomes are required for TR1 cell generation.
  • Fig. S9. Recipient DC and donor IL-27 promote TR1 cell development after experimental BMT.
  • Fig. S10. Eomes and T-bet can be used to identify TR1 cells after clinical BMT.
  • Fig. S11. Proposed cellular and transcriptional regulation of TR1 cell development after BMT.
  • Table S1. Primer sets used for ChIP assays.
  • Reference (58)

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Tabulated data for figures.

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