Science Immunology

Supplementary Materials

Supplementary Material for:

Distinct oxysterol requirements for positioning naïve and activated dendritic cells in the spleen

Erick Lu, Eric V. Dang, Jeffrey G. McDonald, Jason G. Cyster*

*Corresponding author. Email: jason.cyster{at}

Published 7 April 2017, Sci. Immunol. 2, eaal5237 (2017)
DOI: 10.1126/sciimmunol.aal5237

This PDF file includes:

  • Methods
  • Fig. S1. Combined contribution of Cyp27a1 and Ch25h to DCIR2+ DC positioning.
  • Fig. S2. cDC2 marker expression by DCs in EBI2- and ligand-deficient mice.
  • Fig. S3. M12 EBI2 bioassay sensitivity, oxysterol-induced EBI2 internalization, and effect of dietary cholic acid on EBI2 ligand activity in Cyp27a1 KO mice.
  • Fig. S4. Mesenteric LN DCIR2+ DC homeostasis and positioning in Ch25h and Cyp27a1 single- and double-deficient mice.
  • Fig. S5. RNAscope analysis of enzyme expression in the spleen and liver and efficiency of DC ablation.
  • Fig. S6. EBI2 antagonist specificity, method for quantifying DC distribution, and retroviral transduction efficiency of activated T cells.
  • Fig. S7. Characterization of EBI2:ZDC-DTR mixed chimeras.
  • Fig. S8. Activation marker induction and positioning of DCIR2+ DCs and sites of type I IFN action in regulating EBI2 expression and function.
  • Fig. S9. Activation marker induction, positioning, in vivo PE labeling, and enzyme expression of XCR1+ DCs.
  • Fig. S10. Model for na?ve and activated DCIR2+ DC positioning.
  • Fig. S11. Model for type I IFN and Batf3 DC effects on outer T cell zone positioning of activated DCIR2+ DCs.
  • Fig. S12. Sort gating strategy and purity of sorted splenic DCs.
  • References (43, 44)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Tabulated data for Figs. 1 to 7 and figs. S1 to S9.

Files in this Data Supplement: