Science Immunology

Supplementary Materials

Supplementary Material for:

Synovial fibroblast-neutrophil interactions promote pathogenic adaptive immunity in rheumatoid arthritis

Carmelo Carmona-Rivera, Philip M. Carlucci, Erica Moore, Nithya Lingampalli, Hannes Uchtenhagen, Eddie James, Yudong Liu, Kevin L. Bicker, Heidi Wahamaa, Victoria Hoffmann, Anca Irinel Catrina, Paul R.Thompson, Jane H. Buckner, William H. Robinson, David A. Fox, Mariana J. Kaplan*

*Corresponding author. Email: mariana.kaplan{at}nih.gov

Published 14 April 2017, Sci. Immunol. 2, eaag3358 (2017)
DOI: 10.1126/sciimmunol.aag3358

This PDF file includes:

  • Materials and Methods
  • Fig. S1. ACPAs recognize NET peptides.
  • Fig. S2. Skin fibroblasts from patients with psoriasis internalize NETs.
  • Fig. S3. NETs up-regulate type I IFN-inducible genes in FLS.
  • Fig. S4. Endogenous expression of RAGE and TLR9 in FLS.
  • Fig. S5. Up-regulation of MHC II in OA FLS incubated with NETs.
  • Fig. S6. IFN-γ is not detected in NETs or FLS supernatants.
  • Fig. S7. Detection of IL-17A and IL-17B in neutrophil lysate and NETs.
  • Fig. S8. NETs are internalized by a TLR9-dependent and IL-17R–independent mechanism.
  • Fig. S9. FLS do not express HC-gp39 or MPO.
  • Fig. S10. Characterization of vimentin epitope binding to HLA-DRB1*04:04 and T cell clone specificity.
  • Fig. S11. Cytokine profile of cocultures of Aggrecan 225 CD4+ T cells and haplotype-matched FLS.
  • Fig. S12. GK1.5 antibody depletes specifically CD4 T cells but not CD8 T cells.
  • Fig. S13. DRB1*04:01 transgenic mice immunized with FLS loaded with NETs develop in vivo antibody responses to citrullinated peptides.
  • Table S1. Mass spectrometry analysis demonstrates multiple citrullinated peptides in NETs induced with IgM rheumatoid factor.
  • Reference (56)

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Other Supplementary Material for this manuscript includes the following:

  • Source data Excel file
  • Source data blots (.pptx format)

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