Science Immunology

Supplementary Materials

Supplementary Material for:

Resident memory T cells in the skin mediate durable immunity to melanoma

Brian T. Malik, Katelyn T. Byrne, Jennifer L. Vella, Peisheng Zhang, Tamer B. Shabaneh, Shannon M. Steinberg, Aleksey K. Molodtsov, Jacob S. Bowers, Christina V. Angeles, Chrystal M. Paulos, Yina H. Huang, Mary Jo Turk*

*Corresponding author. Email: mary.jo.turk{at}dartmouth.edu

Published 14 April 2017, Sci. Immunol. 2, eaam6346 (2017)
DOI: 10.1126/sciimmunol.aam6346

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Phenotype of CD8 T cells and OT-I cells in vitiligo-affected skin.
  • Fig. S2. IFN-γ production by endogenous TRP-2–specific CD8 T cells in the skin.
  • Fig. S3. Appearance of vitiligo-affected skin grafts on RAG-/- mice.
  • Fig. S4. Phenotype of skin graft–derived CD8 T cells that enter lymph nodes.
  • Fig. S5. CD103 expression by tumor-primed pmel cells.
  • Fig. S6. Absence of CLA on FucT-VII-/- T cells.
  • Fig. S7. Tumor growth kinetics in FucT-VII-/- mice.
  • Fig. S8. Adoptive immunotherapeutic efficacy of CD103-deficient CD8 T cells.
  • Fig. S9. CD8 T cell depletion from the skin by anti-CD8 mAb.
  • Fig. S10. Effects of local skin depigmentation status on tumor protection.
  • Fig. S11. Absence of protection against LLC in mice with vitiligo.
  • Fig. S12. Isotype staining controls for flow cytometry.
  • Fig. S13. Flow cytometry gating strategies.
  • Fig. S14. Representative control staining for immunohistochemistry.
  • Table S1. Primary source data.
  • Reference (30)

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