Science Immunology

Supplementary Materials

Supplementary Material for:

Type I interferon responses drive intrahepatic T cells to promote metabolic syndrome

Magar Ghazarian, Xavier S. Revelo, Mark K. Nøhr, Helen Luck, Kejing Zeng, Helena Lei, Sue Tsai, Stephanie A. Schroer, Yoo Jin Park, Melissa Hui Yen Chng, Lei Shen, June Ann D'Angelo, Peter Horton, William C. Chapman, Diane Brockmeier, Minna Woo, Edgar G. Engleman, Oyedele Adeyi, Naoto Hirano, Tianru Jin, Adam J. Gehring, Shawn Winer,* Daniel A. Winer*

*Corresponding author. Email: shawn.winer{at}utoronto.ca (S.W.); dan.winer{at}uhn.ca (D.A.W.)

Published 21 April 2017, Sci. Immunol. 2, eaai7616 (2017)
DOI: 10.1126/sciimmunol.aai7616

This PDF file includes:

  • Experimental Procedures
  • Fig. S1. Metabolic parameters and hepatic T cell subsets of HFD-fed and ob/ob mice.
  • Fig. S2. Cytokine production by T cell subsets in the livers of ob/ob mice and WT HFD-fed mice, as well as spleens of mice fed a HFD for 16 weeks.
  • Fig. S3. Metabolic parameters and/or hepatic T cell populations of WT mice fed HFD for shorter duration (10 weeks) or longer duration (32 weeks).
  • Fig. S4. Surface TCRVβ expression in hepatic and splenic T cells in HFD- or NCD-fed mice.
  • Fig. S5. Correlative analyses of human intrahepatic mononuclear cell subsets to BMI or percent HbA1c.
  • Fig. S6. Metabolic and liver function parameters of HFD-fed CD8−/− mice and expression of IFN-responsive genes in immune cells.
  • Fig. S7. Additional metabolic assessment and T cell subset analysis of IFNαR1−/− mice.
  • Fig. S8. Bacterial products drive hepatic IFN-I production and T cell inflammation.
  • Table S1. Fluorophore-conjugated antibodies used for flow cytometry.
  • Table S2. Primer sets used for RT-PCR.

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Other Supplementary Material for this manuscript includes the following:

  • Table S3 (Microsoft Excel format). Raw data.

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