Science Immunology

Supplementary Materials

Supplementary Material for:

The lung is a host defense niche for immediate neutrophil-mediated vascular protection

Bryan G. Yipp,* Jung Hwan Kim, Ronald Lima, Lori D. Zbytnuik, Björn Petri, Nick Swanlund, May Ho, Vivian G. Szeto, Tamar Tak, Leo Koenderman, Peter Pickkers, Anton T. J. Tool, Taco W. Kuijpers, Timo K. van den Berg, Mark R. Looney, Matthew F. Krummel, Paul Kubes*

*Corresponding author. Email: bgyipp{at}ucalgary.ca (B.G.Y.); pkubes{at}ucalgary.ca (P.K.)

Published 28 April 2017, Sci. Immunol. 2, eaam8929 (2017)
DOI: 10.1126/sciimmunol.aam8929

This PDF file includes:

  • Fig. S1. Flow cytometry gating strategy for neutrophils.
  • Fig. S2. Cell surface versus intracellular CD11b after LPS.
  • Fig. S3. Dual antibody labeling reveals neutrophil anatomic compartmentalization in the lung using flow cytometry.
  • Fig. S4. CD11b deficiency has a greater impact on rapid LPS-mediated crawling in the lung.
  • Fig. S5. CD11b expression in lung neutrophils from wild-type and Myd88-deficient mice.
  • Fig. S6. Rapid LPS-mediated neutrophil adhesion does not require transcription or translation.
  • Fig. S7. Rapid LPS-induced up-regulation of CD11b requires abl kinase.
  • Legends for movies S1 to S5

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Baseline neutrophil behaviors in unstimulated mouse lung in vivo.
  • Movie S2 (.mov format). LPS initiates a rapid neutrophil crawling behavior in vivo.
  • Movie S3 (.mov format). LPS-stimulated rapid neutrophil crawling requires CD11b in vivo.
  • Movie S4 (.mov format). Abl inhibition impairs rapid lung neutrophil CD11b expression after LPS in vivo.
  • Movie S5 (.mov format). Neutrophils in the lung rapidly respond and capture bloodstream microbial invaders.
  • Source Data (Microsoft Excel format)

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