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Supplementary Materials

Supplementary Material for:

Complement factor P is a ligand for the natural killer cell–activating receptor NKp46

Emilie Narni-Mancinelli,* Laurent Gauthier, Myriam Baratin, Sophie Guia, Aurore Fenis, Ala-Eddine Deghmane, Benjamin Rossi, Patrick Fourquet, Bertrand Escalière, Yann M. Kerdiles, Sophie Ugolini, Muhamed-Kheir Taha, Eric Vivier*

*Corresponding author. Email: narni{at}ciml.univ-mrs.fr (E.N.-M.); vivier{at}ciml.univ-mrs.fr (E.V.)

Published 28 April 2017, Sci. Immunol. 2, eaam9628 (2017)
DOI: 10.1126/sciimmunol.aam9628

This PDF file includes:

  • Fig. S1. Characterization of soluble recombinant NCR-Fc fusion molecules.
  • Fig. S2. B12 cells express a ligand for NKp46.
  • Fig. S3. 27A1.7 mAb recognizes JAM1, but JAM1 is not a direct ligand for NKp46.
  • Fig. S4. Characterization of soluble recombinant CFP-HIS fusion molecules.
  • Fig. S5. Native CFP oligomers bind to NKp46-Fc.
  • Fig. S6. B12 cell–derived CFP activates NKp46 reporter cells.
  • Fig. S7. CFP does not bind to T cells.
  • Fig. S8. CFP stimulation does not induce classical NK cell effector functions.
  • Fig. S9. Impact of anti-NK1.1 and anti-aGM1 treatments on NKp46+ ILC subsets.
  • Fig. S10. Impact of anti-aGM1 treatment on Nm infection.
  • Fig. S11. Therapeutic benefit of CFP delivery for Nm infection control in the absence of NKp46+ ILCs or NKp46.
  • Table S1. Affinity/avidity of CFP for the NKp46 receptor.
  • Table S2. List of genes modulated upon NKp46 stimulation.
  • Table S3. List of genes modulated upon CFP stimulation.

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