Science Immunology

Supplementary Materials

Supplementary Material for:

Virion incorporation of integrin α4β7 facilitates HIV-1 infection and intestinal homing

Christina Guzzo, David Ichikawa, Chung Park, Damilola Phillips, Qingbo Liu, Peng Zhang, Alice Kwon, Huiyi Miao, Jacky Lu, Catherine Rehm, James Arthos, Claudia Cicala, Myron S. Cohen, Anthony S. Fauci, John H. Kehrl, Paolo Lusso*

*Corresponding author. Email: plusso{at}niaid.nih.gov

Published 12 May 2017, Sci. Immunol. 2, eaam7341 (2017)
DOI: 10.1126/sciimmunol.aam7341

This PDF file includes:

  • Fig. S1. Schematic representation and validation of the immunomagnetic virion capture assay.
  • Fig. S2. Role of HIV-1 Gag and Env components in virion incorporation of integrin α4β7.
  • Fig. S3. Effect of RA treatment on cell surface expression and virion incorporation of different lymphocyte markers.
  • Fig. S4. Correlation between α4β7 cell surface expression and α4β7 incorporation into HIV-1 progeny virions.
  • Fig. S5. Validation of engineered α4β7+ and α4β7 HIV-1 THRO viral stocks.
  • Fig. S6. Validation of engineered α4β7+ and α4β7 HIV-1 BaL pseudovirus stocks.
  • Legend for movie S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). A three-dimensional image reconstruction showing α4β7+ HIV-1 virions captured along the endothelial surface of HEVs in Peyer?s patches.
  • Raw Data (Microsoft Excel format). Raw data tables with statistical analyses.

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