Science Immunology

Supplementary Materials

Supplementary Material for:

Human thymoproteasome variations influence CD8 T cell selection

Takeshi Nitta, Yuta Kochi, Ryunosuke Muro, Yoshihiko Tomofuji, Tadashi Okamura, Shigeo Murata, Harumi Suzuki, Takayuki Sumida, Kazuhiko Yamamoto, Hiroshi Takayanagi*

*Corresponding author. Email: takayana{at}m.u-tokyo.ac.jp

Published 2 June 2017, Sci. Immunol. 2, eaan5165 (2017)
DOI: 10.1126/sciimmunol.aan5165

This PDF file includes:

  • Fig. S1. Generation of Psmb11 mutant mice by the CRISPR/Cas9 method.
  • Fig. S2. Normal development of TECs in Psmb11 mutant mice.
  • Fig. S3. Detection of the β5t protein in cTECs from Psmb11 mutant mice.
  • Fig. S4. Reduced CD8 T cell development in Psmb11G49S/A208T heterozygous mice.
  • Fig. S5. Identification of the N-terminus of the β5tG49S protein.
  • Fig. S6. Altered TCR-Vα/Vβ usage in Psmb11G49S mice.
  • Fig. S7. The entire immunoblots for each protein shown in Figs. 1E (A) and 2B (B).
  • Table S1. Genetic variants of the genes encoding the core proteasome subunits from the United States.
  • Table S2. Genetic variants of the genes encoding the core proteasome subunits from Japan.
  • Table S3. Allele frequency and genotype count of Psmb11 variations obtained from the Exome database.
  • Table S4. Summary of TCR repertoire sequencing.
  • Table S5. Two representative TCRs obtained from repertoire sequencing analysis.

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Other Supplementary Material for this manuscript includes the following:

  • Table S6 (Microsoft Excel format). Raw data.

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