Science Immunology

Supplementary Materials

Supplementary Material for:

Citrullination of NF-κB p65 promotes its nuclear localization and TLR-induced expression of IL-1β and TNFα

Bo Sun, Nishant Dwivedi, Tyler J. Bechtel, Janet L. Paulsen, Aaron Muth, Mandar Bawadekar, Gang Li, Paul R. Thompson, Miriam A. Shelef, Celia A. Schiffer, Eranthie Weerapana, I-Cheng Ho*

*Corresponding author. Email: iho{at}partners.org

Published 9 June 2017, Sci. Immunol. 2, eaal3062 (2017)
DOI: 10.1126/sciimmunol.eaal3062

This PDF file includes:

  • Fig. S1. Impacts of BB-Cl on neutrophils.
  • Fig. S2. Functional redundancy between PAD2 and PAD4.
  • Fig. S3. Immunocytochemistry of dHL60 cells.
  • Fig. S4. Preparation of native and cit–GST-p65 for MS.
  • Fig. S5. 4R-K mutant does not affect the interaction between p65 and PAD4.
  • Fig. S6. Citrullination of p65 does not attenuate its binding to the IL-1β NF-κB site.
  • Table S1. Spectral counts and charge states for citrullinated peptides identified in gel band extractions of citrullinated and native GST-p65 via LC-MS/MS.
  • Table S2. Sequence of primers used in real-time PCR.

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