Science Immunology

Supplementary Materials

Supplementary Material for:

Memory-phenotype CD4+ T cells spontaneously generated under steady-state conditions exert innate TH1-like effector function

Takeshi Kawabe,* Dragana Jankovic, Shuko Kawabe, Yuefeng Huang, Ping-Hsien Lee, Hidehiro Yamane, Jinfang Zhu, Alan Sher,* Ronald N. Germain,* William E. Paul

*Corresponding author. Email: takeshi.kawabe{at}nih.gov (T.K.); asher{at}niaid.nih.gov (A.S.); rgermain{at}niaid.nih.gov (R.N.G.)

Published 16 June 2017, Sci. Immunol. 2, eaam9304 (2017)
DOI: 10.1126/sciimmunol.aam9304

This PDF file includes:

  • Fig. S1. MP cell conversion in LN.
  • Fig. S2. Slow and fast homeostatic proliferations generate na?ve and MP cells, respectively.
  • Fig. S3. Naïve CD4+ T cells generate MP cells independently of commensal antigens.
  • Fig. S4. CD5 expression is maintained in MP cell population.
  • Fig. S5. MP cell proliferation is less dependent on TCR signaling.
  • Fig. S6. T-bet expression on MP cells in PC depends on IL-12.
  • Fig. S7. Relative contribution of NK, MP CD4+ T, NKT, γδT, and MP CD8+ T cells to early IFN-γ production during T. gondii infection.
  • Fig. S8. MP cells established in Rag γc KO mice exert protective function in the absence of cognate antigen recognition.
  • Fig. S9. Gating strategy.

Download PDF

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). RNA-seq data.
  • Table S2 (Microsoft Excel format). Raw data sets.

Files in this Data Supplement: