Science Immunology

Supplementary Materials

Supplementary Material for:

Preferential induction of cross-group influenza A hemagglutinin stem–specific memory B cells after H7N9 immunization in humans

Sarah F. Andrews,* M. Gordon Joyce, Michael J. Chambers, Rebecca A. Gillespie, Masaru Kanekiyo, Kwanyee Leung, Eun Sung Yang, Yaroslav Tsybovsky, Adam K. Wheatley, Michelle C. Crank, Jeffrey C. Boyington, Madhu S. Prabhakaran, Sandeep R. Narpala, Xuejun Chen, Robert T. Bailer, Grace Chen, Emily Coates, Peter D. Kwong, Richard A. Koup, John R. Mascola, Barney S. Graham, Julie E. Ledgerwood, Adrian B. McDermott*

*Corresponding authors. Email: adrian.mcdermott{at}nih.gov (A.B.M.); sarah.andrews2{at}nih.gov (S.F.A.)

Published 14 July 2017, Sci. Immunol. 2, eaan2676 (2017)
DOI: 10.1126/sciimmunol.aan2676

This PDF file includes:

  • Fig. S1. Immunization protocols and response.
  • Fig. S2. Ig repertoire analysis of heterosubtypic B cells.
  • Fig. S3. Analysis of Ig heavy chain junction of VH1-2/VH3-53 class antibodies.
  • Fig. S4. B cell response of volunteers that received both the H5N1 and H7N9 vaccine.
  • Fig. S5. Gating strategy for analyzing and sorting HA-specific B cells by flow cytometry.
  • Table S1. List of influenza strain HA proteins.
  • Table S2. Demographic information for donors used for single-cell sorting and Ig sequencing.
  • Table S3. Genetic characteristics of Ig heavy and light chains of expressed and characterized mAbs.
  • Table S4. IC50 microneutralization titers.
  • Table S5. IC80 neutralization titers by pseudovirus entry inhibition assay.
  • Table S6. Crystallographic data collection and refinement statistics.

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